Anti-phospho-TDP-43 (pS409/pS410) antibody
Description
Application Data
Description
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An antibody against TAR DNA binding protein 43 (TDP-43) phosphorylated at serines 409 and 410. TDP-43 binds RNA and is associated with neurodegenerative disorders.
Application Data
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Anti-phospho-TDP-43 (pS409/pS410) antibody (crb2005319) Western blot analysis of recombinant full length TDP-43 with in vitro phosphorylation using casein kinase 1, blotted using batch number N00 and M100. The arrow points to phosphorylated full length TDP-43 Western blot image provided courtesy of Dr. Benjamin Ryskeldi-Falcon, MRC-LMB.
Anti-phospho-TDP-43 (pS409/pS410) antibody (crb2005319) Western blot analysis of recombinant full length wt, S409A, S410A and S409A + S410A TDP-43 with in vitro phosphorylation using casein kinase 1, blotted using batch number N00 and M100. The arrow points to phosphorylated full length TDP-43 Western blot image provided courtesy of Dr. Benjamin Ryskeldi-Falcon, MRC-LMB.
Anti-phospho-TDP-43 (pS409/pS410) antibody (crb2005319) Western blot analysis of sarkosyl-insoluble TDP-43 extraceted from postmortem brain tissue of an individual who had frontotemporal dementia with TDP-43 pathology type A (FTLD-TDP-A), blotted using batch number N00 and M100. The arrow points to phosphorylated full length and N-terminally truncated TDP-43. Western blot image provided courtesy of Dr. Benjamin Ryskeldi-Falcon, MRC-LMB.
| Catalogue number | crb2005319 |
|---|---|
| Antibody | Anti-phospho-TDP-43 (pS409/pS410) antibody |
| Antigen Peptide | KLH conjugated synthetic peptide crb1200552 |
| Protein ID | UniProtKB: Q13148 |
| Aliases | TAR DNA-binding protein 43, TARDBP, TDP43 |
| Cross-Reactivity | Human |
| Target Protein Species | Human |
| Host Species | Anti-Rabbit |
| Antibody Type | Polyclonal |
| Concentration | N00 Glycine 1mg/ml, M100 Glycine 0.4mg/ml |
| Validation | ELISA (1:1000), WB (0.2 µg/mL) |
| Target | phosphorylated TDP-43 (pS409/pS410) |
| Storage Stabilisers | The product should be stored at -20°C for for short term storage and long term storage. Avoid repeated freeze/ thaw cycles. |
| Family | heterogeneous nuclear ribonucleoprotein (hnRNP) family |
| Specificity | Protein |
| Post-translational Modification | Phosphorylation |
| Storage | The product should be stored at -20°C for for short term storage and long term storage. Avoid repeated freeze/ thaw cycles. |
| References | Jo et al., (2020). The role of TDP-43 propagation in neurodegenerative diseases: integrating insights from clinical and experimental studies. Exp Mol Med 52: 1652. Doi: 10.1038/s12276-020-00513-7. Prasad et al., (2019). Molecular mechanisms of TDP-43 misfolding and pathology in amyotrophic lateral sclerosis. Front. Mol. Neurosci., 12: 25. doi: 10.3389/fnmol.2019.00025. |
TAR DNA binding protein 43 (TDP-43) is a highly conserved protein that binds RNA via its two RNA recognition motifs RRM1 and RRM2, thereby regulating RNA processing. Binding regulates mRNA stability by recruiting CNOT7/CAF1 deadenylase on mRNA 3’UTR leading to poly(A) tail deadenylation and thus, shortening.
TDP-43 aggregates are linked to numerous neurodegenerative diseases, including amyotrophic lateral sclerosis (ALS), frontotemporal dementia (FTD), Alzheimer’s disease (AD), and limbic predominant age-related TDP-43 encephalopathy (LATE).
This TDP-43 antibody is raised against the phosphorylated form at serines 409 and 410, this modification is associated with the pathological aggregated TDP-43. We also have an antibody available for the unphosphorylated form of TDP-43.