Anti-NR2B (C-ter) antibody
Recognises the C-terminal region of the NR2B subunit of NMDA receptors
Western blot analysis of rat brain preparation.
Lane 1: Rat brain preparation (10µg).
Secondary: Goat anti-rabbit IgG conjugated to HRP 1:2000.
|Antibody||Anti-NR2B (C-ter) antibody|
|Antigen Peptide||KLH conjugated synthetic peptide crb1200338e|
|Protein ID||UniProtKB - Q13224|
|Aliases||GRIN2B, GluN2B, MRD6, NMDAR2B, NR2B, hNR3, EIEE27, glutamate ionotropic receptor NMDA type subunit 2B, NMDA NR2B Subunit|
|Cross-Reactivity||Human, Rat, Mouse|
|Target Protein Species||Human, Rat, Mouse|
|Validation||1:1000 (WB), 1:1000 (ELISA)|
|Storage Stabilisers||This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4°C for short term storage and -20°C for long term storage. Avoid repeated freeze/ thaw cycles.|
|Family||Glutamate-gated ion channel family|
|Disease Area||Neurodegenerative diseases|
|Storage||This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4°C for short term storage and -20°C for long term storage. Avoid repeated freeze/ thaw cycles.|
Li, Y., Sun, W., Han, S., Li, J., Ding, S., Wang, W. and Yin, Y. (2016). IGF-1-Involved Negative Feedback of NR2B NMDA Subunits Protects Cultured Hippocampal Neurons Against NMDA-Induced Excitotoxicity. Mol Neurobiol, 54(1), 684-696. PMID: 26758454
Liao, G. Y., Wagner. D. A., Hsu, M. H., Leonard, J. P. (2001). Evidence for direct protein kinase-C mediated modulation of N-methyl-D-aspartate receptor current. Mol Pharmacol. 59(5), 960-964. PMID: 11306676
Wang, Y., Han, S., Han, R., Su, Y. and Li, J. (2017). Propofol-induced downregulation of NR2B membrane translocation in hippocampus and spatial memory deficits of neonatal mice. Brain and Behav, 7(7), e00734. PMID: 28729940
N-methyl-D-aspartate receptors (NMDARs) are ligand-gated ionotropic glutamate receptors that mediate excitatory synaptic transmission and play important roles in many aspects of nervous system function including: synaptic plasticity; learning and memory; neuronal development and circuit formation. NMDARs have been implicated in various neuronal disorders. NMDARs are heteromers consisting of an obligate NR1 subunit and most commonly one or two kinds of NR2 subunits or occasionally NR3 subunits.
NR2B is the predominant subunit found in the postnatal brain, however over time the number of NRA2 subunits increase and eventually outnumber NR2B subunits in a process known as the NR2B-NR2A developmental switch. The greater ratios of the NR2B subunit in post-natal brains leads to NMDA receptors which remain open longer compared to those with more NR2A subunits, this may be related to the greater memory abilities in the immediate postnatal period compared to late in life. NR2B improves synaptic plasticity and memory when over-expressed in neurones.
The involvement of NMDAR in the central nervous system (CNS) has become a focus area for neurodegenerative diseases such as Alzheimer’s disease, epilepsy and ischemic neuronal cell death.