This service provides the highest quality of target specific custom polyclonal antibodies that we offer. There are many steps to generating a custom antibody and care and attention to every detail and many years of refining the process at every stage have given CRB its enviable track record. We bring both a chemical and biological perspective to antigen design, while our first-rate project management and purification strategies help guarantee a high success rate for the production of polyclonal antibodies.
We offer an a la carte menu for greater success and flexibility which typically follows the stages: Antigenic prediction — peptide synthesis — carrier conjugation — immunisation — ELISA screening — Affinity Purification.
In addition, we offer validation by western blot analysis, modification (Fab fragmentation) and labelling (fluorescent, biotin or enzyme) of antibodies.
Major benefits of ordering this service are:
- Free Consultation
- Expert advice on the vital first step of antigen selection
- From either your target protein or peptide sequence
- Cleaner product: Peptide antigens are synthesised in-house and purified by HPLC
- Peace of mind: Peptides are conjugated in-house and monitored for combination
- High specificity and yield of purified antibody due to the quality of the peptide affinity media prepared in-house
- We use only high quality animals housed in UK Home Office, USDA & NIH compliant facilities
- A variety of species and screening strategies employed for best approach
- ISO9001:2015 Quality Accreditation
- Close involvement between CRB and client’s own goals to steer project
- Fully-managed data from antibody generation programme
- Data available in ‘real time’ via my account, or in format specified by researcher
A Record of Success
At CRB we pride ourselves on the quality of our antibodies. According to customer feedback:
- 100% of antibodies raised against peptide recognise the peptide antigen
- 70% of these antibodies react with protein by Western Blot
- 50% of these antibodies react with protein in immunohistochemistry