Peptide synthesis

PNA Synthesis

PNAs are synthetic analogues of DNA based upon an N-(2-aminoethyl)glycine pseudopeptide backbone. The bases (corresponding to A,G,C,T) are attached to the backbone via a methylene carbonyl linker and the resulting PNA oligomers are able to form very stable complexes with complementary DNA and RNA. They also have the great advantage of being resistant to nucleases (and proteases) which makes them potentially ideal reagants for antisense and antigene applications. Labelled PNAs also have applications in molecular diagnostics and as probes in microarrays.

Through an agreement with Panagene Inc, CRB can offer high purity custom PNA oligomers in quantities ranging from 50 nmole to grams to the UK market. PNAs are synthesized on solid phase using Panagene’s patented BTS chemistry and Panagene has the worldwide exclusive license obtained from the Copenhagen Inventor Group (CIG), the inventors¹ for custom PNA. CRB can also offer custom PNA labelled with a variety of reporter molecules such as fluorescent dyes and Biotin.

Although PNAs work well in cell free systems, poor cellular uptake can limit their use in the regulation of gene expression in cell culture and in vivo. The conjugation of PNA to peptides, to lipophilic molecules and to cell-specific receptor ligands offers new ways to surmount this problem. CRB are ideally placed to advise on and supply such conjugates.

As UK distributor for Panagene Inc (South Korea) CRB can offer the following products and services through our online catalogue DISCOVERY Peptides – simply follow the links below:

Contact CRB directly here for PNA related enquiries.

¹ Peptide Nucleic Acids (PNA) were discovered by Nielsen, Egholm, Berg and Buchardt in 1991.