We are often asked by customers, “how successful are your antibodies?” and our general reply is that many of our customers are core antibody groups within large pharmaceutical companies (in-house antibody specialists with many years of generating antibodies themselves) and they have chosen CRB to custom generate their antibodies for more than ten years. So from this we deduce we must have a good success rate and be easy to work with.
Discovery research is highly confidential and a lot of our clients want us to respect their privacy so we are not allowed to mention who we support. However, if you wish to work with us for the first time and would like some reassurance of our success rate, then we’d be happy to put you in touch with one or two of our long-standing customers who are happy to discuss how it feels working with CRB. Please contact us to sort this out for you.
CRB did produce commercial antibodies from 1985 until 1994 when the catalogue business was sold to Sigma-Genosys and therefore any searches made on Cambridge Research Biochemicals will bring up lots of commercial antibodies used and cited in that period. From 1994 onwards the company focused on custom produced antibodies, mainly against surrogate peptides. In 2016 the company launched its new catalogue business, DISCOVERY® (read more at our History page).
Here are some examples of CRB-produced antibodies in recent years which have been published and kindly notified to us by customers. Some of the cited antibodies are available to purchase in CRB’s DISCOVERY® Antibodies catalogue and their information is provided below:
- RORα is a critical checkpoint for T cell and ILC2 commitment in the embryonic thymus. McKenzie, A. et al, MRC Laboratory of Molecular Biology (2021). Nature Immunology, 22, 166–178. DOI: 10.1038/s41590-020-00833-w Anti-T2A antibody (crb2005269)
- Fumarate Metabolic Signature for the Detection of Reed Syndrome in Humans.
Casey, R. et al, University of Cambridge (2020). Clinical Cancer Research, 26(2), 391-396. DOI: 10.1158/1078-0432.CCR-19-1729. Anti-2SC antibody (crb2005017)
- Secondary Renal Neoplasia Following Chemotherapy or Radiation in Pediatric Patients. Gupta, S. et al, Mayo Clinic (2020). Human Pathology, DOI: 10.1016/j.humpath.2020.07.014 Anti-2SC antibody (crb2005017)
- Systematic molecular and clinical analysis of uterine leiomyomas from fertile-aged women undergoing myomectomy. Vahteristo, P. et al, University of Helsinki (2020). Human Reproduction, 35(10), 2237–2244. DOI: 10.1093/humrep/deaa187
- Human Antibodies that slow erythrocyte invasion potentiate malaria-neutralizing antibodies.
Alanine, D. et al, University of Oxford (2019). Cell, 178(1), 216-228. DOI: 10.1016/j.cell.2019.05.025.
- The major secreted protein of the whipworm parasite tethers to matrix and inhibits interleukin-13 function.
Bancroft, A. et al, University of Manchester (2019). Nature Communications, 10, 2344. DOI: 10.1074/jbc.RA118.006230.
- SDHA gain-of-function engages inflammatory mitochondrial retrograde signaling via KEAP1-Nrf2.Burgener, A. et al, University of Basil (2019). Nat. Immunol, 20(10), 1311-1321. DOI: 10.1038/s41590-019-0482-2 Anti-2SC antibody (crb2005017)
- In vivo localization of human acetylcholinesterase-derived species in a β-sheet conformation at the core of senile plaques in Alzheimer’s disease.
Jean, L. et al, School of Pathology University of Oxford (2019). The Journal of Biological Chemistry, 294, 6253-6272. DOI: 10.1074/jbc.RA118.006230.
- LncRNA EPR controls epithelial proliferation by coordinating Cdkn1a transcription and mRNA decay response to TGF-β.
Rossi, M. et al, Gene Expression Regulation Laboratory, Genova (2019). Nature Communications, 10, 1969. DOI: 10.1038/s41467-019-09754-1.
- The α2δ-like Protein Cachd1 Increases N-type Calcium Currents and Cell Surface Expression and Competes with α2δ-1.
Dahimene, S. et al, University College London (2018). Cell reports, 25, 1610-1621. DOI: 10.1016/j.celrep.2018.10.033
- Species-Specific Detection of C. difficile Using Targeted Antibody Design.
Lawry, B. et al, Newcastle University (2018). Anal. Chem., 90(22), 13475-13482. DOI: 10.1021/acs.analchem.8b03349.
- Development of reverse genetics systems and investigation of host response antagonism and reassortment potential for Cache Valley and Kairi viruses, two emerging orthobunyaviruses of the Americas.
Kohl, A. et al, MRC-University of Glasgow Centre for Virus Research (2018). PLOS Neglected Tropical Diseases, 12(10), p.e0006884. DOI: 10.1371/journal.pntd.0006884.
Anti-Cache valley virus (CVV) nucleocapsid antibody (crb2005018), Anti-Kairi virus (KRIV) nucleocapsid antibody (crb2005080)
- Bioinformatic analysis of meningococcal Msf and Opc to inform vaccine antigen design.
Andreae, C. et al., University of Bristol (2018). PLOS ONE, 13(3), p.e0193940. DOI: 10.1371/journal.pone.0193940.
- γ-TuRC Heterogeneity Revealed by Analysis of Mozart1
Conduit, P. et al., University of Cambridge (2018). Current Biology, 28(14), 2314-2323. DOI: 10.1016/j.cub.2018.05.044.
Anti-Grip71 antibody (crb2005268)
- Murine leukemia virus p12 tethers the capsid-containing pre-integration complex to chromatin by binding directly to host nucleosomes in mitosis.
Bishop, K. et al., Francis Crick Institute London (2018). PLOS Pathogens, 14(6), p.e1007117. DOI: 10.1371/journal.ppat.1007117.
- NADH Shuttling Couples Cytosolic Reductive Carboxylation of Glutamine with Glycolysis in Cells with Mitochondrial Dysfunction.
Frezza, C. et al., University of Cambridge MRC (2018). Molecular Cell, 69(4), 581-593.e7. DOI: 10.1016/j.molcel.2018.01.034.
- Gene expression of AvBD6-10 in broiler chickens is independent of AvBD6, 9, and 10 peptide potency.
Hall, J. et al., Newcastle University (2018). Veterinary Immunology and Immunopathology, 202, 31-40. DOI: 10.1016/j.vetimm.2018.06.007.
- Structural basis for membrane tethering by a bacterial dynamin-like pair.
Liu, J., Noel, J. and Low, H., Imperial College London (2018). Nature Communications, 9(1).
- Wilms Tumor 1b defines a wound-specific sheath cell subpopulation associated with notochord repair.
- The integrated stress response regulates BMP signalling through effects on translation.
Marciniak, S. et al., University of Cambridge MRC (2018). BMC Biology, 16(1). DOI: 10.1186/s12915-018-0503-x
- Mitochondria-derived ROS activate AMP-activated protein kinase (AMPK) indirectly
Murphy, M. et al, MRC Mitochondrial Biology Unit, University of Cambridge (2018) Journal of Biological Chemistry, 9, 3345. DOI:10.1038/s41467-018-05523-8
- aPKC controls endothelial growth by modulating c-Myc via FoxO1 DNA-binding ability.Riddell, M. et al, Max Planck Institute for Heart and Lung Research, (2018). Nature Comm, 9(1). 5357. doi: 10.1038/s41467-018-07739-0. Anti-phospho-FoxO1 (pS218) antibody (crb2005226)
- SUMO conjugation to the pattern recognition receptor FLS2 triggers intracellular signalling in plant innate immunity.
- Evaluation of a novel antibody to define histone 3.3 G34R mutant brain tumours.
Layfield, R. et al., University of Nottingham (2017). Acta Neuropathologica Communications, 5(1). DOI: 10.1186/s40478-017-0449-1.
Anti-Histone H3 (G34R) antibody (crb2005185d)
- Vaccinia virus A11 is required for membrane rupture and viral membrane assembly.
Pénard, E. et al, Centre for Innovation and Technological Research, Ultrastructural Bio-imaging, France (2017). Cellular Microbiology, 19(10), p.e12756. DOI: 10.1111/cmi.12756.
- Analysis of the Interplay of protein biogenesis factors at the ribosome exit site now reveals new role for NAC.
Nyathi, Y. et al, University of Manchester (2015). 210(2), 287-301. 10.1083/jcb.201410086.
- Multigene manipulation of photosynthetic carbon assimilation increases CO2 fixation and biomass yield in tobacco.
Raines, C. et al, University of Essex (2015). Journal of Experimental Botony, 66 (13), pp. 4075-4090. DOI: 10.1093/jxb/erv204.
- Generation of functionally distinct isoforms of PTBP3 by alternative splicing and translation initiation.
Whitfield et al, University of Cambridge & The Babraham Institute (2015). Nucleic Acids Res. 43 (11), 5586-5600. DOI: 10.1093/nar/gkv429.
- Nek5 promotes centrosome integrity in interphase and loss of centrosome cohesion in mitosis.
Fry, A. et al, University of Leicester (2015). J. Cell. Biol., 209 (3), 339-348. DOI: 10.1083/jcb.201412099.
- Analysis of the redox oscillations in the circadian clockwork.
O’Neill, J. et al, MRC-Laboratory of Molecular Biology (2015). Methods Enzymol., 552, 185-210. DOI: 10.1016/bs.mie.2014.10.007.
Anti-Peroxiredoxin SO2/3 antibody (crb2005004)
- Osteoactivin Induces Transdifferentiation of C2C12 Myoblasts into Osteoblasts.
Sondag, G. et al, Northeast Ohio Medical University (2014). J. Cell. Physiol, 229(7), 955-966. DOI: 1371/journal.pone.0107211.
- LeoA, B and C from Enterotoxigenic Escherichia coli (ETEC) Are Bacterial Dynamins.
Löwe, J. et al, Structural Studies Division-MRC Laboratory of Molecular Biology (2014). PLOS ONE, 9(9), e107211. DOI: 1371/journal.pone.0107211.
- Heightened immune response to autocitrullinated Porphyromonas gingivalis peptidylarginine deiminase: a potential mechanism for breaching immunologic tolerance in rheumatoid arthritis.
Quirke, A. et al, The Kennedy Institute of Rheumatology (2014). Ann Rheum Dis, 73(1), 263–269. DOI:1136/annrheumdis-2012-202726.
- Investigation of soluble and Tm-CTLA-4 isoforms in serum and microvesicles.
Esposito, L. et al, Cambridge Institute for Medical Research (2014). Journal of Immunology, 193(2), 889-900. DOI:10.4049/jimmunol.1303389
- Molecular Basis and Regulation of OTULIN-LUBAC Interaction.
Komander. D. et al, Medical Research Council Laboratory of Molecular Biology (2014). Molecular Cell, 54(3), 335-348. DOI:10.1016/j.molcel.2014.03.018
Anti-OTULIN antibody (crb2005228)
- Biochemical Analysis of the Plasmodium falciparum Erythrocyte-binding Antigen-175 (EBA175)-Glycophorin-A Interaction: implications for vaccine design.
Wright, G. et al, Cell Surface Signalling Laboratory, Wellcome Trust Sanger Institute (2013). The Journal of Biological Chemistry, 288(45), 32106–32. DOI:10.1074/jbc.M113.484840
- Coiled-coil domain containing protein 124 is a novel centrosome and midbody protein that interacts with the Ras-guanine nucleotide exchange factor 1B and is involved in cytokinesis.
Telkoparan, P. et al, Department of Molecular Biology and Genetics, Bilkent University (2013). PLoS One, 19; 8 (7), e69289. DOI: 1371/journal.pone.0069289.
- OTULIN Antagonizes LUBAC Signalling by Specifically Hydrolyzing Met1-Linked Polyubiquitin.
Komander, D. et al, MRC Laboratory of Molecular Biology (2013). Cell, 6(153),1312–1326. DOI: 1016/j.cell.2013.05.014
- A Systematic Investigation of Differential Effects of Cell Culture Substrates on the Extent of Artifacts in Single-Molecule Tracking.
Needham, S. et al, Science and Technology Facilities Council (2012). PLoS ONE, 7(9), e45655. DOI:10.1371/journal.pone.0045655.
- A Role for Rab7 in the Movement of Secretory Granules in Cytotoxic T Lymphocytes.
Daniele, T. et al, The Cambridge Institute for Medical Research (2011). Traffic, 12(7), 902–911. DOI:10.1111/j.1600-0854.2011.01194.x.
- Impaired tissue growth is mediated by checkpoint kinase 1 (CHK1) in the integrated stress response.
- Regulation of Insulin Receptor Substrate 1 Pleckstrin Homology Domain by Protein Kinase C: Role of Serine 24 Phosphorylation.
Nawaratne, R. et al, University of Cambridge (2006). Molecular Endocrinology, 20(8), 1838-1852. DOI:10.1210/me.2005-0536.
- Coordinate Regulation of the Mother Centriole Component Nlp by Nek2 and Plk1 Protein Kinases.
Fry, A. et al, University of Leicester (2005). Molecular and Cellular Biology, 25(4), 1309–1324. DOI: 10.1128/MCB.25.4.1309-1324.2005
- Marine Fish Egg Hydration Is Aquaporin-Mediated.
Cerda, J. et al (2005). Science, 307 (5709), 545. Anti-SaAQP1o antibody. DOI: 10.1186/1471-2148-8-259
- A mouse sperm decapacitation factor receptor is phosphatidylethanolamine-binding protein 1.
Fraser, L. et al, King’s College London (2005). Reproduction,130, 497-508. Specific anti-PEBP-1 antibodies. DOI:1530/rep.1.00792.