An antibody against TAR DNA binding protein 43 (TDP-43). TDP-43 binds RNA and is associated with neurodegenerative disorders.
Anti-TDP-43 antibody (crb2005321) Western blot analysis of recombinant SUMO-tagged full length wt and R293A TDP-43 (0.5µg) without (-) or with (+) in vitro citrullination using peptidyl-arginine deiminase (PAD4) blotted using batch number N01 and N03 (0.2µg/mL). The asterisk indicates truncated TDP-43. Western blot image provided courtesy of Dr. Benjamin Ryskeldi-Falcon, MRC-LMB.
|Antigen Peptide||KLH conjugated synthetic peptide crb12000555|
|Protein ID||UniProtKB: Q13148|
|Aliases||TAR DNA-binding protein 43, TARDBP, TDP43|
|Target Protein Species||Human|
|Concentration||N01 0.2mg/ml, N03 0.3mg/ml|
|Storage Stabilisers||The product should be stored at -20°C for for short term storage and long term storage. Avoid repeated freeze/ thaw cycles.|
|Family||heterogeneous nuclear ribonucleoprotein (hnRNP) family|
|Storage||The product should be stored at -20°C for for short term storage and long term storage. Avoid repeated freeze/ thaw cycles.|
Jo et al., (2020). The role of TDP-43 propagation in neurodegenerative diseases: integrating insights from clinical and experimental studies. Exp Mol Med 52: 1652. Doi: 10.1038/s12276-020-00513-7.
Prasad et al., (2019). Molecular mechanisms of TDP-43 misfolding and pathology in amyotrophic lateral sclerosis. Front. Mol. Neurosci., 12: 25. doi: 10.3389/fnmol.2019.00025.
TAR DNA binding protein 43 (TDP-43) is a highly conserved protein that binds RNA via its two RNA recognition motifs RRM1 and RRM2, thereby regulating RNA processing. Binding regulates mRNA stability by recruiting CNOT7/CAF1 deadenylase on mRNA 3’UTR leading to poly(A) tail deadenylation, and shortening.
TDP-43 aggregates are linked to numerous neurodegenerative diseases, including amyotrophic lateral sclerosis (ALS), frontotemporal dementia (FTD), Alzheimer’s disease (AD), and limbic predominant age-related TDP-43 encephalopathy (LATE).
This TDP-43 antibody detects the native form of the protein without modifications. We also have an antibody available to the phosphorylated form, serines 409 and 410.