Anti-TDP-43 antibody
Description
Application Data
Description
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An antibody against TAR DNA binding protein 43 (TDP-43). TDP-43 binds RNA and is associated with neurodegenerative disorders.
Application Data
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Anti-TDP-43 antibody (crb2005321) Western blot analysis of recombinant SUMO-tagged full length wt and R293A TDP-43 (0.5µg) without (-) or with (+) in vitro citrullination using peptidyl-arginine deiminase (PAD4) blotted using batch number N01 and N03 (0.2µg/mL). The asterisk indicates truncated TDP-43. Western blot image provided courtesy of Dr. Benjamin Ryskeldi-Falcon, MRC-LMB.
| Catalogue number | crb2005321 |
|---|---|
| Antibody | Anti-TDP-43 antibody |
| Antigen Peptide | KLH conjugated synthetic peptide crb12000555 |
| Protein ID | UniProtKB: Q13148 |
| Aliases | TAR DNA-binding protein 43, TARDBP, TDP43 |
| Cross-Reactivity | Human |
| Target Protein Species | Human |
| Host Species | Anti-Rabbit |
| Antibody Type | Polyclonal |
| Concentration | N01 0.2mg/ml, N03 0.3mg/ml |
| Validation | ELISA (1:1000) |
| Target | TDP-43 |
| Storage Stabilisers | The product should be stored at -20°C for for short term storage and long term storage. Avoid repeated freeze/ thaw cycles. |
| Family | heterogeneous nuclear ribonucleoprotein (hnRNP) family |
| Specificity | Protein |
| Storage | The product should be stored at -20°C for for short term storage and long term storage. Avoid repeated freeze/ thaw cycles. |
| References | Jo et al., (2020). The role of TDP-43 propagation in neurodegenerative diseases: integrating insights from clinical and experimental studies. Exp Mol Med 52: 1652. Doi: 10.1038/s12276-020-00513-7. Prasad et al., (2019). Molecular mechanisms of TDP-43 misfolding and pathology in amyotrophic lateral sclerosis. Front. Mol. Neurosci., 12: 25. doi: 10.3389/fnmol.2019.00025. |
TAR DNA binding protein 43 (TDP-43) is a highly conserved protein that binds RNA via its two RNA recognition motifs RRM1 and RRM2, thereby regulating RNA processing. Binding regulates mRNA stability by recruiting CNOT7/CAF1 deadenylase on mRNA 3’UTR leading to poly(A) tail deadenylation, and shortening.
TDP-43 aggregates are linked to numerous neurodegenerative diseases, including amyotrophic lateral sclerosis (ALS), frontotemporal dementia (FTD), Alzheimer’s disease (AD), and limbic predominant age-related TDP-43 encephalopathy (LATE).
This TDP-43 antibody detects the native form of the protein without modifications. We also have an antibody available to the phosphorylated form, serines 409 and 410.