Alexa Fluor® 488 Anti-Phospho-GluR2 (pS880) antibody
Description
Application Data
Description
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Recognises the GluR2 subunit of AMPAR with a phosphorylated serine at position 880. Antibody is conjugated to Alexa Fluor® 488 fluorescent dye
Application Data
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Data Sheet Material Safety Data Sheet (MSDS)Catalogue number crb2115005e Antibody Alexa Fluor® 488 Anti-Phospho-GluR2 (pS880) antibody Antigen Peptide KLH conjugated synthetic peptide crb1200275e Protein ID UniProtKB - Q14416 Aliases GRIA2, GLUR2, GLURB, GluA2, GluR-K2, HBGR2, glutamate ionotropic receptor AMPA type subunit 2 Cross-Reactivity Rat Target Protein Species Rat Host Species Anti-Rabbit Antibody Type Polyclonal Label Labelled with Alexa Fluor® 488 under optimal conditions. Ex: 495nm Em:519nm Concentration 0.25mg/ml Validation ELISA Target phosphorylated GluR2 (pS880) Storage Stabilisers Supplied in PBS containing 0.01% sodium azide. The product should be stored undiluted and protected from prolonged exposure to light. Short term storage between 2-8°C and long term storage below -20°C. Family Glutamate-gated ion channel family. Disease Area Neurodegenerative diseases Specificity PTM - Phosphorylated serine 880 Post-translational Modification Phosphorylation of Serine 880 Storage Supplied in PBS containing 0.01% sodium azide. The product should be stored undiluted and protected from prolonged exposure to light. Short term storage between 2-8°C and long term storage below -20°C. Citations Kohda, K., Kakegawa, W., Matsuda, S., Yamamoto, T., Hirano, H. and Yuzaki, M. (2013). The 2 glutamate receptor gates long-term depression by coordinating interactions between two AMPA receptor phosphorylation sites. PNAS, 110(10), E948-E957. PMID: 23431139
References Kohda, K., Kakegawa, W., Matsuda, S., Yamamoto, T., Hirano, H. and Yuzaki, M. (2013). The 2 glutamate receptor gates long-term depression by coordinating interactions between two AMPA receptor phosphorylation sites. PNAS, 110(10), E948-E957. PMID: 23431139
The α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR) is an ionotropic transmembrane receptor for glutamate found throughout the central nervous system (CNS). AMPARs are composed of four types of subunits: GluR1/GluA1, GluR2/GluA2, GluR3/GluA3, and GluA4 (GluRA-D2), which combine to form heterotetramers.
The GluR2 subunit interacts with several proteins and regulates the lysosomal targeting and degradation of AMPARs. Phosphorylation of AMPARs is often implicated in synaptic plasticity and the pathological signalling seen in several disease states. Phosphorylation of GluR2 on serine 880 is involved in changes of synaptic efficacy where it regulates GluR2 synaptic recycling, AMPA signalling strength and plasticity.
This antibody is conjugated to Alexa Fluor® 488. Alexa Fluor® 488 is a popular bright green fluorescent dye with high pH-stability.