Alexa Fluor® 488 Anti-NR3B antibody
Description
Application Data
Description
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Recognises the NR3B subunit from NMDA receptors with a strong signal and low background noise. Conjugated to Alexa Fluor® 488 fluorescent dye
Application Data
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Data Sheet Material Safety Data Sheet (MSDS)Catalogue number crb2115052e Antibody Alexa Fluor® 488 Anti-NR3B antibody Antigen Peptide KLH conjugated synthetic peptide crb1200339e Protein ID UniProtKB - P11474 Cross-Reactivity Rat, mouse Target Protein Species Rat, mouse Host Species Anti-Rabbit Antibody Type Polyclonal Concentration 0.56mg/mL Validation ELISA Target NR3B Storage Stabilisers Supplied in PBS containing 0.01% sodium azide. The product should be stored undiluted and protected from prolonged exposure to light. Short term storage between 2-8°C and long term storage below -20° Family Glutamate gated ion channel famility Disease Area Neurodegenerative Specificity Protein Storage Supplied in PBS containing 0.01% sodium azide. The product should be stored undiluted and protected from prolonged exposure to light. Short term storage between 2-8°C and long term storage below -20° Citations Ciabarra, A., Sullivan, J., Gahn, L., Pecht, G., Heinemann, S. and Sevarino, K. (1995). Cloning and characterization of chi-1: a developmentally regulated member of a novel class of the ionotropic glutamate receptor family. J Neurosci, 15(10), 6498-6508. PMID: 7472412
Low, C. and Wee, K. (2010). New Insights into the Not-So-New NR3 Subunits of N-Methyl-D-aspartate Receptor: Localization, Structure, and Function. Mol Pharmacol, 78(1), 1-11. PMID: 20363861
Prithviraj, R. and Inglis, F. (2008). Expression of the N-methyl-d-aspartate receptor subunit NR3B regulates dendrite morphogenesis in spinal motor neurons. Neuroscience, 155(1), 145-153. PMID: 18541382
References Ciabarra, A., Sullivan, J., Gahn, L., Pecht, G., Heinemann, S. and Sevarino, K. (1995). Cloning and characterization of chi-1: a developmentally regulated member of a novel class of the ionotropic glutamate receptor family. J Neurosci, 15(10), 6498-6508. PMID: 7472412
Low, C. and Wee, K. (2010). New Insights into the Not-So-New NR3 Subunits of N-Methyl-D-aspartate Receptor: Localization, Structure, and Function. Mol Pharmacol, 78(1), 1-11. PMID: 20363861
Prithviraj, R. and Inglis, F. (2008). Expression of the N-methyl-d-aspartate receptor subunit NR3B regulates dendrite morphogenesis in spinal motor neurons. Neuroscience, 155(1), 145-153. PMID: 18541382
N-methyl-D-aspartate receptors (NMDARs) are ligand-gated ionotropic glutamate receptors that mediate excitatory synaptic transmission and are important for many aspects of nervous system function including: synaptic plasticity; learning and memory; neuronal development and circuit formation. NMDARs have also been implicated in various neuronal disorders. NMDARs are heteromers consisting of an obligate NR1 and most commonly one or two kinds of NR2 subunits or occasionally NR3 subunits.
The NR3 isoform, NR3B, is abundantly expressed in α-motoneurones and at a lower level in the forebrain (including hippocampus, cerebral cortex, caudoputamen, and nucleus accumbens), and cerebellum. mRNA for the NR3B subunit is first detected during the second postnatal week, it then increases and peaks at postnatal day 21. NR3B is a dominant-negative receptor subunit since its presence in NMDA receptors results in a reduction in cellular NMDA receptor activity, calcium permeability, and whole-cell currents.
NR3B plays a role in determining the morphology of dendrites of spinal motor neurones and the proper maturation of motor behaviour. The involvement of NMDAR in the central nervous system (CNS) has become a focus area for neurodegenerative diseases such as Alzheimer’s disease, epilepsy and ischemic neuronal cell death.
This antibody is conjugated to Alexa Fluor® 488. Alexa Fluor® 488 is a popular bright green fluorescent dye with high pH-stability.