Alexa Fluor® 488 Anti-CDK2 antibody
Description
Application Data
Description
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Recognises CDK2, a serine/threonine protein kinase which controls both G1/S and G2/M transitions. Conjugated to Alexa Fluor® 488 fluorescent dye
Application Data
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Data Sheet Material Safety Data Sheet (MSDS)Catalogue number crb2115068e Antibody Alexa Fluor® 488 Anti-CDK2 antibody Antigen Peptide KLH conjugated synthetic peptide crb1200296e Protein ID UnitProtKB - P24941 Aliases Cyclin-dependent kinase 2, CDK2, CDK-2, Cell division protein kinase 2, p33 protein kinase Cross-Reactivity Human, rat, mouse Target Protein Species Human, rat, mouse Host Species Anti-Rabbit Antibody Type Polyclonal Label Labelled with Alexa Fluor® 488 under optimal conditions. Ex: 495nm Em:519nm Concentration 1mg/ml Validation ELISA Target CDK2 Storage Stabilisers Supplied in PBS containing 0.01% sodium azide. The product should be stored undiluted and protected from prolonged exposure to light. Short term storage between 2-8°C and long term storage below -20°C Family Ser/Thr protein kinases (cyclin-dependent) Disease Area Cancer Specificity Protein Storage Supplied in PBS containing 0.01% sodium azide. The product should be stored undiluted and protected from prolonged exposure to light. Short term storage between 2-8°C and long term storage below -20°C Citations Bačević, K., Lossaint, G., Achour, T., Georget, V., Fisher, D. and Dulić, V. (2017). Cdk2 strengthens the intra-S checkpoint and counteracts cell cycle exit induced by DNA damage. Sci Rep, 7(1). PMID: 29044141
Ji, X., Humenik, J., Yang, D. and Liebhaber, S. (2017). PolyC-binding proteins enhance expression of the CDK2 cell cycle regulatory protein via alternative splicing. Nucleic Acids Res, 46(4), 2030-2044. PMID: 29253178
References Bačević, K., Lossaint, G., Achour, T., Georget, V., Fisher, D. and Dulić, V. (2017). Cdk2 strengthens the intra-S checkpoint and counteracts cell cycle exit induced by DNA damage. Sci Rep, 7(1). PMID: 29044141
Ji, X., Humenik, J., Yang, D. and Liebhaber, S. (2017). PolyC-binding proteins enhance expression of the CDK2 cell cycle regulatory protein via alternative splicing. Nucleic Acids Res, 46(4), 2030-2044. PMID: 29253178
Cyclin dependant kinase 2 (CDK2) is a serine/threonine protein kinase which controls both G1/S and G2/M transitions. CDKs along with cyclins and CDK inhibitors regulate cell cycle progression. Specific cyclins activate different CDKs; in early G1, CDK2 pairs with cyclin E to promote entry into the S phase before switching to partner with cyclin A to drive the cell through S phase. Once activated CDKs phosphorylate downstream proteins to initiate signalling cascades. CDK2 phosphorylates and inactivates the RB1 (pRb) tumour suppressor family of proteins.
The activities of the CDKs are tightly controlled. CDK2 activities can be repressed by a set of inhibitors including P21. Dysregulation of the cell cycle and loss of proliferative controls is closely linked to cell transformation and cancer. CDK2 also appears to play a positive role in cell cycle arrest in the DNA damage response (DDR), its inhibition hinders the DDR, and sensitises cells to ionizing radiation, inducing cell death.
This antibody is conjugated to Alexa Fluor® 488. Alexa Fluor® 488 is a popular bright green fluorescent dye with high pH-stability.