C-terminal Sortagging-[Cys(AF680)] amide 0.1mg


  • Description

  • Application Data


Acts as a (oligo)glycine nucleophile in the final steps of a sortagging reaction, which results in the fluorescent dye being attached to the C-terminus of target peptide

See full description

Application Data

Catalogue number crb1101685f
Molecular Weight 1240.3
Sequence (one letter code)


Sequence (three letter code)


Aliase sortase tagging peptide
Modifications Where AF680 is Aurora™ Fluor 680
Purity >95%

Strijbis et al (2012) Protein Ligation in Living Cells Using Sortase. Traffic 13(6) 780 PMID: 22348280

Sinisi et al (2012) Development of an Influenza virus Protein Array Using Sortagging Technology. Bioconjug. Chem. 23(6) 1119 PMID: 22594688

Ton-That et al (2000) Anchoring of Surface Proteins to the Cell Wall ofStaphylococcus aureus. J. Biol. Chem. 275(13) 9876 PMID: 10734144

Manufactured in: United Kingdom
Material Safety Data Sheet (MSDS)

This C-terminal Sortagging peptide acts as a (oligo)glycine nucleophile in the final steps of a sortagging protein labelling reaction. This reaction results in the [Cys(AF680)]- fluorescent moiety being attached to the C-terminus of the target protein or peptide.

A substrate peptide containing the LPXTG motif is recognised and cleaved by the enzyme Sortase A (SrtA) from Staphylococcus aureus. The catalytic cysteine residue in the active site of SrtA, serves as a nucleophile to cleave the peptide bond between threonine and glycine of the substrate peptide. Cleavage results in the formation of a thioacyl intermediate between the substrate peptide and SrtA. This intermediate is then resolved by the N-terminus of this (oligo)glycine nucleophile peptide, resulting in the creation of a new peptide bond that links the substrate peptide to this peptide and its fluorescent dye.  This method of protein labelling is known as sortagging.

This peptide contains the Aurora™ Fluor 680 fluorescent dye.

C-terminal Sortagging-[Cys(AF680)] amide 0.1mg

Bulk Quote

Download Family PDF