C-terminal Sortagging-[Cys(AF680)] acid


  • Description

  • Application Data


C-Terminal Sortagging-[Cys(AF680)] acts as an (oligo)glycine nucleophile in the final steps of a sortagging reaction, which results in the fluorescent dye being attached to the C-terminus of the target peptide. This is the acid form.

See full description

Application Data

Catalogue number crb1101684
Molecular Weight 1241.3
Sequence (one letter code)


Sequence (three letter code)


Purity >95%

Strijbis et al., (2012). Protein Ligation in Living Cells Using Sortase. Traffic, 13(6): 780. PMID: 22348280.

Sinisi et al., (2012). Development of an Influenza virus Protein Array Using Sortagging Technology. Bioconjug. Chem., 23(6): 1119. PMID: 22594688.

Ton-That et al., (2000). Anchoring of Surface Proteins to the Cell Wall of Staphylococcus aureus. J. Biol. Chem., 275(13): 9876. PMID: 10734144.

Manufactured in: United Kingdom
Data Sheet Material Safety Data Sheet (MSDS)

This C-terminal Sortagging peptide acts as an (oligo)glycine nucleophile in the final steps of a sortagging protein labelling reaction. This reaction results in the [Cys(AF680)]- fluorescent moiety being attached to the C-terminus of the target protein or peptide.

A substrate peptide containing the LPXTG motif is recognised and cleaved by the enzyme Sortase A (SrtA) from Staphylococcus aureus. The catalytic cysteine residue in the active site of SrtA serves as a nucleophile to cleave the peptide bond between threonine and glycine of the substrate peptide. Cleavage results in the formation of a thioacyl intermediate between the substrate peptide and SrtA. This intermediate is then resolved by the N-terminus of this (oligo)glycine nucleophile peptide, resulting in the creation of a new peptide bond that links the substrate peptide to this peptide and its fluorescent dye.  This method of protein labelling is known as sortagging.

C-Terminal Sortagging-[Cys(AF680)] contains the AF680 fluorescent dye; AF680 is a bright green dye with excitation at 633 nm, well suited for flow cytometry and imagery. AF680 is particularly photostable, allowing better detection of low abundance conjugates.  C-Terminal Sortagging-[Cys(AF680)] acid is provided here. However, the amide version is also available here.

C-terminal Sortagging-[Cys(AF680)] acid

Cat No.Pack SizePriceQty.
Bulk Quote

Download Family PDF

You may also like…

  • [5-TAMRA]-LPETAG N-terminal Sortagging[5-TAMRA]-LPETAG-amide

    View products
  • C-Terminal Sortagging-[Lys(Biotin]GGG-[K(Biotin)]-amide

    View products
  • GGG-C(AF647) C-Terminal Sortagging

    View products
  • AAA-C(AF647) C-Terminal Sortagging


    View products
  • Biotin-LPETAG N-terminal SortaggingBiotin-LPETAG-amide

    View products
  • [5-TAMRA]-LPETGG N-terminal Sortagging[5-TAMRA]-LPETGG-amide

    View products
  • C-terminal Sortagging-[Cys(AF680)] amide


    View products
  • C-terminal Sortagging-[Cys(Sulfocyanine7)]


    View products
  • C-terminal Sortagging-[Cys(Sulfocyanine3)]


    View products
  • GGG-[K(5-TAMRA)] C-terminal Sortagging


    View products
  • Biotin-LPETGG N-terminal SortaggingBiotin-LPETGG-amide

    View products