Click Chemistry refers to high yielding, selective, carbon-hetero bond formation reactions that are wide in scope and simple to perform. Additionally they create only by products that can be removed without chromatography and can be conducted in in mild aqueous conditions or easily removed solvents. The word “click” describes joining molecular building blocks as though they are two pieces of a seat belt buckle.
Two of the most regularly encountered functional groups for click chemistry are azides and alkynes, and the azide-alkyne cycloaddition has become the most popular click reaction, although several other types of reaction have been identified that fulfill click criteria.
Click chemistry has found wide use in applications such as chemical synthesis, materials science, chemical biology, and drug development and it has also been widely used in biological large molecule labelling. Genetically encoded click chemistry reaction reagents enable researchers to covalently link chemical groups to any site of a target protein with high site selectivity. Bioconjugation via click chemistry is widely used in proteomics and nucleic acid research.