C-terminal Sortagging-[Cys(AF680)] amide


  • Description

  • Application Data


C-Terminal Sortagging-[Cys(AF680)] acts as an (oligo)glycine nucleophile in the final steps of a sortagging reaction, which results in the fluorescent dye being attached to the C-terminus of the target peptide. This is the amide form.

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Application Data

Catalogue number crb1101685
Molecular Weight 1240.3
Sequence (one letter code)


Sequence (three letter code)


Aliase sortase tagging peptide
Modifications Where AF680 is Aurora™ Fluor 680
Purity >95%

Strijbis et al., (2012). Protein Ligation in Living Cells Using Sortase. Traffic, 13(6): 780. PMID: 22348280.

Sinisi et al., (2012). Development of an Influenza virus Protein Array Using Sortagging Technology. Bioconjug. Chem., 23(6): 1119. PMID: 22594688.

Ton-That et al., (2000). Anchoring of Surface Proteins to the Cell Wall of Staphylococcus aureus. J. Biol. Chem., 275(13): 9876. PMID: 10734144.

Manufactured in: United Kingdom
Data Sheet Material Safety Data Sheet (MSDS)

This C-terminal Sortagging peptide acts as an (oligo)glycine nucleophile in the final steps of a sortagging protein labelling reaction. This reaction results in the [Cys(AF680)]- fluorescent moiety being attached to the C-terminus of the target protein or peptide.

A substrate peptide containing the LPXTG motif is recognised and cleaved by the enzyme Sortase A (SrtA) from Staphylococcus aureus. The catalytic cysteine residue in the active site of SrtA serves as a nucleophile to cleave the peptide bond between threonine and glycine of the substrate peptide. Cleavage results in the formation of a thioacyl intermediate between the substrate peptide and SrtA. This intermediate is then resolved by the N-terminus of this (oligo)glycine nucleophile peptide, resulting in the creation of a new peptide bond that links the substrate peptide to this peptide and its fluorescent dye.  This method of protein labelling is known as sortagging.

C-Terminal Sortagging-[Cys(AF680)]  peptide contains the AF680 fluorescent dye; AF680 is a bright green dye with excitation at 633 nm, well suited for flow cytometry and imagery. AF680 is particularly photostable, allowing better detection of low abundance conjugates.  C-Terminal Sortagging-[Cys(AF680)] amide is provided here. However, the acid version is also available here.

C-terminal Sortagging-[Cys(AF680)] amide

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