C-terminal Sortagging-[Cys(AF680)] amide
GGG-[C(AF680)]-amide
Description
Application Data
Description
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C-Terminal Sortagging-[Cys(AF680)] acts as an (oligo)glycine nucleophile in the final steps of a sortagging reaction, which results in the fluorescent dye being attached to the C-terminus of the target peptide. This is the amide form.
Application Data
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Catalogue number crb1101685 Molecular Weight 1240.3 Sequence (one letter code) GGG-[C(AF680)]-amide
Sequence (three letter code) H-Gly-Gly-Gly-[Cys(AF680)]-NH2
Aliase sortase tagging peptide Modifications Where AF680 is Aurora™ Fluor 680 Purity >95% References Strijbis et al., (2012). Protein Ligation in Living Cells Using Sortase. Traffic, 13(6): 780. PMID: 22348280.
Sinisi et al., (2012). Development of an Influenza virus Protein Array Using Sortagging Technology. Bioconjug. Chem., 23(6): 1119. PMID: 22594688.
Ton-That et al., (2000). Anchoring of Surface Proteins to the Cell Wall of Staphylococcus aureus. J. Biol. Chem., 275(13): 9876. PMID: 10734144.
Manufactured in: United Kingdom This C-terminal Sortagging peptide acts as an (oligo)glycine nucleophile in the final steps of a sortagging protein labelling reaction. This reaction results in the [Cys(AF680)]- fluorescent moiety being attached to the C-terminus of the target protein or peptide.
A substrate peptide containing the LPXTG motif is recognised and cleaved by the enzyme Sortase A (SrtA) from Staphylococcus aureus. The catalytic cysteine residue in the active site of SrtA serves as a nucleophile to cleave the peptide bond between threonine and glycine of the substrate peptide. Cleavage results in the formation of a thioacyl intermediate between the substrate peptide and SrtA. This intermediate is then resolved by the N-terminus of this (oligo)glycine nucleophile peptide, resulting in the creation of a new peptide bond that links the substrate peptide to this peptide and its fluorescent dye. This method of protein labelling is known as sortagging.
C-Terminal Sortagging-[Cys(AF680)] peptide contains the AF680 fluorescent dye; AF680 is a bright green dye with excitation at 633 nm, well suited for flow cytometry and imagery. AF680 is particularly photostable, allowing better detection of low abundance conjugates. C-Terminal Sortagging-[Cys(AF680)] amide is provided here. However, the acid version is also available here.