C-terminal Sortagging-[Cys(AF680)] amide
C-Terminal Sortagging-[Cys(AF680)] acts as an (oligo)glycine nucleophile in the final steps of a sortagging reaction, which results in the fluorescent dye being attached to the C-terminus of the target peptide. This is the amide form.
Catalogue number crb1101685 Molecular Weight 1240.3 Sequence (one letter code)
Sequence (three letter code)
Aliase sortase tagging peptide Modifications Where AF680 is Aurora™ Fluor 680 Purity >95% References
Strijbis et al., (2012). Protein Ligation in Living Cells Using Sortase. Traffic, 13(6): 780. PMID: 22348280.
Sinisi et al., (2012). Development of an Influenza virus Protein Array Using Sortagging Technology. Bioconjug. Chem., 23(6): 1119. PMID: 22594688.
Ton-That et al., (2000). Anchoring of Surface Proteins to the Cell Wall of Staphylococcus aureus. J. Biol. Chem., 275(13): 9876. PMID: 10734144.
Manufactured in: United Kingdom
This C-terminal Sortagging peptide acts as an (oligo)glycine nucleophile in the final steps of a sortagging protein labelling reaction. This reaction results in the [Cys(AF680)]- fluorescent moiety being attached to the C-terminus of the target protein or peptide.
A substrate peptide containing the LPXTG motif is recognised and cleaved by the enzyme Sortase A (SrtA) from Staphylococcus aureus. The catalytic cysteine residue in the active site of SrtA serves as a nucleophile to cleave the peptide bond between threonine and glycine of the substrate peptide. Cleavage results in the formation of a thioacyl intermediate between the substrate peptide and SrtA. This intermediate is then resolved by the N-terminus of this (oligo)glycine nucleophile peptide, resulting in the creation of a new peptide bond that links the substrate peptide to this peptide and its fluorescent dye. This method of protein labelling is known as sortagging.
C-Terminal Sortagging-[Cys(AF680)] peptide contains the AF680 fluorescent dye; AF680 is a bright green dye with excitation at 633 nm, well suited for flow cytometry and imagery. AF680 is particularly photostable, allowing better detection of low abundance conjugates. C-Terminal Sortagging-[Cys(AF680)] amide is provided here. However, the acid version is also available here.