Anti-SARS-CoV-2 Spike protein antibody
Recognises SARS-CoV-2 Spike protein polybasic cleavage site
Anti-SARS-CoV-2 Spike protein antibody (crb2005280) K98
A. SDS PAGE and Coomassie stain and B. western blot analysis of 500ng Spike S1 Recombinant protein.
Lane 1: SARS-Cov-2 Spike S1 protein
Lane 2: SARS-Cov Spike S1 protein
Lane 3: MERS Spike S1 protein
Expected sizes: SARS-Cov-2 spike S1
|Antibody||Anti-SARS-CoV-2 Spike protein antibody|
|Antigen Peptide||KLH conjugated synthetic peptide crb1200514e|
|Aliases||Coronavirus, SARS-CoV-2, Covid-19|
|Target Protein Species||Virus|
|Target||SARS-CoV-2 Spike protein|
|Storage Stabilisers||This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4°C for short term storage and -20°C for long term storage. Avoid repeated freeze/ thaw cycles.|
|Specificity||Recognises SARS-CoV-2 Spike protein polybasic cleavage site|
|Storage||This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4°C for short term storage and -20°C for long term storage. Avoid repeated freeze/ thaw cycles.|
Byrnes et al., (2020). Competitive SARS-CoV-2 Serology Reveals Most Antibodies Targeting the Spike Receptor-Binding Domain Compete for ACE2 Binding. mSphere. 5(5): e00802-20. PMID: 32938700
Wang et al., (2020). Molecular simulation of SARS-CoV-2 spike protein binding to pangolin ACE2 or human ACE2 natural variants reveals altered susceptibility to infection. J Gen Virol. 101(9): 921. PMID: 32538738
The SARS-CoV2 is a novel coronavirus identified as the cause of the coronavirus disease 2019 (COVID-19) pandemic that began in Wuhan, China in 2019. SARS-CoV-2 can cause severe diseases such as pneumonia, acute respiratory distress syndrome (ARDS), sepsis, septic shock or multiple organ failure, particularly among the elderly and immunocompromised patients. SARS-CoV-2 virus particles are round, elliptic and often pleomorphic in shape and a diameter of approximately 60–140 nm. The SARS-CoV2 genome encodes four essential structural proteins including the spike (S) glycoprotein, nucleocapsid (N) protein, membrane (M) protein, and the envelope (E) protein, and also several accessory proteins.
The SARS-CoV-2 spike protein is present on the outside of the virus particles and is able to bind to angiotensin-converting enzyme II (ACE2) present on the host cells. The C-terminal receptor binding domain (RBD) of the spike protein binds to the N-terminal peptidase M2 domain of ACE2. This receptor binding results in internalisation of the virus-receptor complex and is therefore the mechanism of entry of SARS-CoV-2 into host cells.