Purified antibodies are characterised by ELISA against the immunising peptide over a range of dilutions up to 1/100000 and compared with the crude antisera. From this the antibody titre (antibody dilution at half maximum response) can be determined. The antibodies eluted from the affinity column at low and high pH are analysed separately. The antibody concentration is determined from the uv absorbance at 280nm.
Further characterisation of the purified antibodies can be carried out by Western blotting if a source of the target protein is provided (purified protein, cell or tissue extract). In some cases we are able to offer immunocytochemical screening of purified antibodies through an external collaboration.