Biotinylation reagents

Biotinylation reagents are popularly used for labelling compounds and covalent coupling because they work over a large range of temperatures and pH and are proteolysis-resitant. The process of biotinylation is attaching biotin to proteins and other macromolecules, quickly and specifically, without changing the usual function of the molecule. Biotinylated compounds can be conjugated with azides, carboxylic acids, amines, thiols, and carbohydrates. Biotin-labelled peptides are widely used for affinity purifications and solid-phase immunoassays because of how easily biotin binds to avidin and streptavidin.