Recognises SUMO protein from Arabidopsis in Western blots with a strong signal and low background noise
SUMO conjugation to FLS2 is flg22 dependant and required for innate immunity in Arabidopsis. FLS2 is rapidly SUMOylated after flg22 treatment. Immunoprecipitation (IP: αGFP) was carried out with anti-GFP beads from total protein derived from two independent transgenic lines expressing proFLS2::FLS2-GFP (fls2) treated either with MgCl2 or 1 µM flg22 for 10 min. Immunoblots were probed with anti-GFP (IB: αGFP) or anti-SUMO1/2 (IB: αSUMO1) antibodies. Col-0 was used as a negative control.
|Aliases||Small Ubiquitin-like Modifier 1|
|Cross-Reactivity||Arabidopsis, Rice, wheat, tomato, barley|
|Target Protein Species||Arabidopsis, Rice, wheat, tomato, barley|
|Storage Stabilisers||The product should be stored at -20°C for short term storage and long term storage. Avoid repeated freeze/ thaw cycles.|
|Storage||The product should be stored at -20°C for short term storage and long term storage. Avoid repeated freeze/ thaw cycles.|
Orosa et al. Nat Commun. 2018 Dec 5;9(1):5185. doi: 10.1038/s41467-018-07696-8. PMID: 30518761
Conti et al., (2008). Small ubiquitin-like modifier proteases OVERLY TOLERANT TO SALT1 and -2 regulate salt stress responses in Arabidopsis. Plant Cell. 20(10):2894. PMID: 18849491
SMALL UBIQUITIN-LIKE MODIFIER (SUMO) protein is a small protein which can be conjugated to target proteins, a process known as SUMOylation. SUMOylation is a key post translational modification in plants that is involved in a wide array of biological processes ranging from development to environmental sensing. SUMOylation acts in a similar way to ubiqutination in that SUMOlayed proteins are targeted for degredation in the proteasome.
SUMO conjugation to its protein target involves an enzyme cascade involving activation of the SUMO polypeptide by SUMO protease and ligation of the SUMO polypeptide to lysine residues in target proteins. The removal of SUMO occurs by deconjugating SUMO protease enzymes. Plants have two SUMO E3 ligases, AtHPY2 and AtSIZ1, encoded by the Arabidopsis genome. SUMOylation is reversible due to the presence of SUMO proteases that possess de-conjugation activity and are able to cleave SUMO from target proteins.