Anti-SLC15A4 antibody
Description
Application Data
Description
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Recognises SLC15A4 with high affinity. SLC15A4 is a membrane bound histidine/oligopeptide transporter involved in autoimmunity
Application Data
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| Catalogue number | crb2005113 |
|---|---|
| Antibody | Anti-SLC15A4 antibody |
| Antigen Peptide | KLH conjugated synthetic peptide crb1200375e |
| Protein ID | UniProtKB - Q8N697 |
| Aliases | Solute carrier family 15 member 4, Peptide transporter 4, Peptide/histidine transporter 1, hPHT1 |
| Cross-Reactivity | Human |
| Target Protein Species | Human |
| Host Species | Anti-Rabbit |
| Antibody Type | Polyclonal |
| Concentration | 0.2mg/ml |
| Validation | 1:1000 (WB), 1:1000 (ELISA) |
| Target | SLC15A4 |
| Storage Stabilisers | This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4°C for short term storage and -20°C for long term storage. Avoid repeated freeze/ thaw cycles. |
| Family | PTR2/POT transporter family |
| Disease Area | Proton oligopeptide cotransporter |
| Specificity | Protein |
| Storage | This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4°C for short term storage and -20°C for long term storage. Avoid repeated freeze/ thaw cycles. |
| References | Griffith, A., Zaidi, A., Pietro, A., Hadiono, M., Yang, J., Davis, R. and Popkin, D. (2018). A requirement for slc15a4 in imiquimod-induced systemic inflammation and psoriasiform inflammation in mice. Sci Rep, 8(1). PMID: 30262916
Kobayashi, T., Tsutsui, H., Shimabukuro-Demoto, S., Yoshida-Sugitani, R., Karyu, H., Furuyama-Tanaka, K., Ohshima, D., Kato, N., Okamura, T. and Toyama-Sorimachi, N. (2017). Lysosome biogenesis regulated by the amino-acid transporter SLC15A4 is critical for functional integrity of mast cells. Int Immunol, 29(12), 551-566. PMID: 29155995
Yamashita, T., Shimada, S., Guo, W., Sato, K., Kohmura, E., Hayakawa, T., Takagi, T. and Tohyama, M. (1997). Cloning and Functional Expression of a Brain Peptide/Histidine Transporter. J Biol Chem, 272(15), 10205-10211. PMID: 9092568 |
Solute carrier family member 15A4 (SLC15A4) is a lysosome-resident, proton-coupled histidine/oligopeptide transporter that has 12 membrane-spanning regions. SLC15A4 is expressed in haematopoietic lineage cells, including dendritic cells and B-cells, where it transports certain amino acids and oligopeptides to the cytosol, along with a proton from the inside of the late endosome/lysosome. SLC15A4 is critical for the production of type I interferon (IFN-I). SLC15A4’s transporter activity is required to optimize pH and other conditions required for signalling in the late endosomes/lysosomes as well as in specialised lysosomes know as secretory granules present in mast cells. SLC15A4-mediated regulation of signalling in these compartments is an integral part of innate immune responses and may play a role in autoimmune diseases.