Anti-RAMP2 antibody
Description
Application Data
Description
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Recognises C-terminal region of RAMP2, a receptor accessory protein in Western blots and immunohistochemistry with a strong signal and low background noise
Application Data
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Immunofluorescence image of Human Embryonic Kidney cells (HEK293S) transiently transfected with either pcDNA3.1 alone or HA-hCLR and FLAG-hRAMP2 for 48h
| Catalogue number | crb2005160 |
|---|---|
| Antibody | Anti-RAMP2 antibody |
| Antigen Peptide | KLH conjugated synthetic peptide crb1200418e |
| Protein ID | UniProtKB - O60895 |
| Cross-Reactivity | Human |
| Target Protein Species | Human |
| Host Species | Rabbit |
| Antibody Type | Polyclonal |
| Concentration | 1mg/ml |
| Validation | 1:40 (WB), 1:200 (IF), 1:1000 (ELISA) |
| Target | RAMP2 |
| Storage Stabilisers | This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4°C for short term storage and -20°C for long term storage. Avoid repeated freeze/ thaw cycles. |
| Family | RAMP family |
| Storage | This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4°C for short term storage and -20°C for long term storage. Avoid repeated freeze/ thaw cycles. |
| References | McLatchie et al., (1998). RAMPs regulate the transport and ligand specificity of the calcitonin-receptor-like receptor. Nature.393(6683): 333. PMID: 9620797
Weston et al., (2015). Modulation of Glucagon Receptor Pharmacology by Receptor Activity-modifying Protein-2 (RAMP2). J Biol Chem.290(38): 23009. PMID: 26198634 |
Receptor activity-modifying protein 2 (RAMP2) is a receptor accessory protein that alters the pharmacology of G-protein coupled receptors (GPCRs). RAMP2 associates with GPCRs to alter ligand specificity and mediate receptor trafficking. RAMP2 forms a receptor complex with calcitonin receptor-like receptor (CRLR) known as the calcitonin gene-related protein (CGRP) receptor. RAMP2 is responsible for the transport of this receptor to the plasma membrane.
RAMPs also associate with other family B G-protein coupled receptors including the calcitonin receptor, the vasoactive intestinal polypeptide/pituitary AC-activating peptide 1 (VPAC1) receptor, and the glucagon receptor (GCGR) where they modulate ligand and G-protein selection.