Anti-OOEP antibody
Description
Application Data
Description
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FLOPED/OOEP is an abundant protein in the oocyte cytoplasm, the absence of this protein leads to early embryonic arrest in both mice and humans
Application Data
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Anti-OOEP antibody validated using mouse oocytes isolated from 8 week old FVB/N mice. Oocytes were fixed in 2% PFA (+ 100 mM Hepes
| Catalogue number | crb2005295 |
|---|---|
| Antibody | Anti-OOEP antibody |
| Antigen Peptide | KLH conjugated synthetic peptide crb1200531e |
| Protein ID | UniProtKB - Q9CWE6 |
| Aliases | Oocyte-expressed protein homolog, OOEP , Factor located in oocytes permitting embryonic development, FLOPED, Oocyte- and embryo-specific protein 19, mOEP19 |
| Cross-Reactivity | Mouse |
| Target Protein Species | Mouse |
| Host Species | Rabbit |
| Antibody Type | Polyclonal |
| Concentration | 1 mg/ml Glycine eluate (L26) |
| Validation | WB (1:1000), ELISA (I:1000), IF (1:1000) |
| Target | mouse OOEP |
| Storage Stabilisers | This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4°C for short term storage and -20°C for long term storage. Avoid repeated freeze/ thaw cycles. |
| Family | KH domain RNA binding proteins |
| Super Family | KHDC1 family |
| Disease Area | Oocyte biology, early developmental biology, Infertility, recurrent hydatidiform mole formation |
| Specificity | Recognises N-terminus |
| Storage | This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4°C for short term storage and -20°C for long term storage. Avoid repeated freeze/ thaw cycles. |
| References | Li et al., (2008). A Subcortical Maternal Complex Essential for Pre-implantation Mouse Embryogenesis. Dev Cell. 15(3): 416. PMID: 18804437
Tashiro et al., (2010). Maternal‐effect gene Ces5/Ooep/Moep19/Floped is essential for oocyte cytoplasmic lattice formation and embryonic development at the maternal‐zygotic stage transition. Genes to cells. 15 (8). 813. |
Factor located in oocytes permitting embryonic development (FLOPED)/OOEP, along with, MATER, TLE6 and Filia, forms the subcortical maternal complex (SCMC). The SCMC assembles during oocyte growth and is essential for zygotes to progress beyond the first embryonic cell divisions.
Floped expression is seen specifically in growing and mature oocytes and in early embryos, and is localised in the subcortex region.
FLOPED/OOEP is conserved across mammals, and mutations in the Floped gene have been linked to female infertility. Floped knockout mutant eggs lack the SCMC and these embryos do not progress beyond cleavage stage development. Therefore FLOPED/OOEP protein seems to have an important function around the zygotic stage and in the subsequent development of the preimplantation embryos. Floped/Ooep gene is considered a typical maternal‐effect gene.