Anti-β-actin antibody
Description
Application Data
Description
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Detects β-actin proteins in Western blots and immunohistochemistry. Used as an internal control for experiments
Application Data
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Western blot analysis of mouse brain preparation.
Lane 1: Mouse brain preparation (24µg)
| Catalogue number | crb2005000 |
|---|---|
| Antibody | Anti-β-actin antibody |
| Antigen Peptide | KLH conjugated synthetic peptide crb1200274e |
| Protein ID | UniProtKB - P60709 |
| Aliases | ACTB, BRWS1, PS1TP5BP1, Beta-actin, β actin, β-actin, βactin |
| Cross-Reactivity | Human, Rat, Mouse |
| Target Protein Species | Human, Rat, Mouse |
| Host Species | Rabbit |
| Antibody Type | Polyclonal |
| Label | Unconjugated |
| Concentration | 1mg/ml |
| Validation | WB/ELISA |
| Target | β-actin |
| Storage Stabilisers | This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4°C for short term storage and -20°C for long term storage. Avoid repeated freeze/ thaw cycles. |
| Family | Actin |
| Super Family | Internal cellular protein |
| Disease Area | Cell motility, structure and integrity |
| Specificity | Protein |
| Storage | This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4°C for short term storage and -20°C for long term storage. Avoid repeated freeze/ thaw cycles. |
| References | Dugina, V., Khromova, N., Rybko, V., Blizniukov, O., Shagieva, G., Chaponnier, C., Kopnin, B. and Kopnin, P. (2015). Tumor promotion by γ and suppression by β non-muscle actin isoforms. Oncotarget, 6(16). PMID: 26008973 |
Antibody that detects β-actin proteins. Actin is a highly conserved structural protein that forms microfilaments, one of the main structural components of the cell cytoskeleton and is therefore found at high concentrations in all cell types. Actin may also function as a tumour suppresser. β-actin is one of the three main actin isotypes and is a non-muscle cytoskeletal actin. β-actin is relatively stable and appears to remain at constant levels regardless of experimental treatment, it is generally used as an internal control for experiments.