Anti-ɣH2AX (pS139) antibody
Recognises phosphorylated histone H2AX in Western blots and immunohistochemistry. Localises to sites of DNA damage to initiate DNA repair mechanisms
|Antibody||Anti-ɣH2AX (pS139) antibody|
|Antigen Peptide||KLH conjugated synthetic peptide crb1200386e|
|Protein ID||UniProtKB - P16104|
|Aliases||Phosphorylated Histone H2AX (pS139)|
|Target Protein Species||Human, mouse|
|Validation||1:1000 WB, 1:800 IF, 1:1000 ELISA|
|Target||gamma H2AX (pS139)|
|Storage Stabilisers||This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4°C for short term storage and -20°C for long term storage. Avoid repeated freeze/ thaw cycles.|
|Disease Area||Histones, DNA damage|
|Post-translational Modification||Phosphorylation of Serine 139|
|Storage||This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4°C for short term storage and -20°C for long term storage. Avoid repeated freeze/ thaw cycles.|
Feng et al., (2017). H2AX facilitates classical non-homologous end joining at the expense of limited nucleotide loss at repair junctions. Nucleic Acids Res, 45(18), 10614-10633. PMID: 28977657
Kuo and Yang (2008). Gamma-H2AX – a novel biomarker for DNA double-strand breaks. In Vivo, 22(3), 305-9. PMID: 18610740
Antibody which recognises the phosphorylated form of the H2A histone variant H2AX. H2AX is rapidly phosphorylated on serine 139 in response to DNA double strand breaks (DSBs). DSBs occur as part of the natural process of meiosis, and in response to external stimuli and mutagens. In either form, DSBs pose a huge threat to genome integrity and must be repaired quickly and accurately.
In mammalian cells, repair of DSBs is carried out primarily by either homologous recombination (HR) or non-homologous end joining (NHEJ). Upon DNA damage, phosphorylated H2AX, termed ‘γH2AX’, quickly accumulates and initiates a DNA damage signalling cascade at the DSB site. γH2AX also alters the chromatin at the DSB site to form an area accessible to the protein interactions and modifications required for DSB repair.
Anti-ɣH2AX can be seen to stain DSBs shortly after formation to form bright foci associated with the chromatin at the DSB site.