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CRB Discovery
  In this issue
  Lanthanide Labelling:
Europium Chelates for Time-Resolved Fluorescence
Spectrum™ Peptides:
CRB offers Custom Peptides labelled with the most
comprehensive set of Fluorescent dyes available
Fluorescent Dye Selector:
Our interactive online dye selection wheel

  Lanthanide Labelling  
Cambridge Research Biochemicals (CRB) is pleased to offer a new Custom Labelling service using the QuickAllAssay™ Europium Chelate Protein Labelling Kit to label proteins (antibodies) with Europium chelate for inclusion in to Time-Resolved Fluorescence Energy Transfer (TR-FRET); Quenched Resonance Energy Transfer (QRET) assays and Separation based enhanced time-resolved fluorescence assays.

QuickAllAssay™ kits are a range of complete, ready-to-use reagents for labelling of proteins, antibodies, and small molecules with a Europium chelate. Ideal for TR FRET (Time-Resolved Fluorescence Resonance Energy Transfer) and QRET (Quenched Resonance Energy Transfer assays) and for separation based enhanced time-resolved fluorescence assays.

QRET assays - CRB is also pleased to offer a number of complete assays kits based on QRET (Quenched Resonance Energy Transfer assays)
  Separation based TRF assays offer a superior alternative to traditonal ELISA technology
Spectrum PeptidesSpectrum Peptides
CRB specialises in the synthesis of fluorescently-labelled peptides and antibodies. With licences from leading dye manufacturers such as Life Technologies (Invitrogen-Molecular Probes), GE Healthcare (Amersham Biosciences), Biosearch Technologies, Licor Biosciences and Atto-Tec, we have the largest selection of dyes available for peptide labelling of any company in the world, enabling us to offer the best dye for your particular application.
Available Proprietary Dyes Include:
  • Life Technologies Dyes: Alexa Fluor®*, Bodipy®*, Oregon Green®
  • GE Healthcare: CyDyes™*
  • Atto-Tec: ATTO dyes
  • Cyanagen: Chromis dyes
  • Biosearch Technologies: CAL Fluor®, Quasar®
  • Licor Biosciences: IR Dyes®*­
* Requires payment of a royalty.

Non-proprietary dyes: FAM, TAMRA, EDANS, Mca, FITC
Technical Excellence:
  • Scalability: 0.5 mg to grams
  • Dye can be attached to N-terminus, C-terminus or side-chain of the peptide using amino-, thiol-, or click-reactive derivatives
  • Optimised strategy for synthesis of FRET peptides to minimise background fluorescence
  • ISO9001:2008 certified
Streamlined operation allows delivery in 3-4 weeks. Most of the commonly used dyes are kept in stock to ensure rapid turnaround.

Read More

Fluorescent Dye Selector
Our fluorescent dye selector will take you through a step by step process to help you choose the best dye for your application.
Use our online fluorescent dye selector at
which provides a list of suitable dyes matching your desired wavelength and the necessary technical data to enable you to select the optimum dye for your application.
  Fluorescent Dye Selector
We offer dyes compatible with all commonly used excitation sources. The most frequently requested are listed, together with appropriate quencher for FRET applications. If you require a peptide labelled with a dye not listed, please enquire as we have access to many more.
Need to know more? Contact us by visiting our website or email us for information on peptides
Need to know more? Contact us by visiting our website or email us for information on peptides
Need to know more? Contact us by visiting our website or email us for information on peptides
Customer References

* denotes CRB’s customer
Alexa Fluor 488 labelled peptide
Generation of transgenic plantain (Musa spp.) with resistance to plant pathogenic nematodes
Roderick H, Tripathi L, Babirye A, Wang D*, Tripathi J, Urwin PE, Atkinson HJ
Molecular Plant Pathology 2012, 13(8):842-851
Alexa 488, Alexa 547, Atto 647N labelling of mouse EGF and affibodies
Optics clustered to output unique solutions: a multi-laser facility for combined single molecule and ensemble microscopy.
Clarke DT, Botchway SW, Coles BC, Needham SR*, Roberts SK*, Rolfe DJ, Tynan CJ, Ward AD, Webb SE, Yadav R, Zanetti-Domingues L, Martin-Fernandez ML*.
Mol Cell Biol. 2011 Jun;31(11):2241-52
Atto 488 and Alexa 546 labelling of mouse EGF (Epidermal Growth Factor)
Human-EGFR aligned on the plasma membrane adopts key features of Drosophila-EGFR asymmetry.
Tynan CJ, Roberts SK*, Rolfe DJ, Clarke DT, Loeffler HH, Kästner J, Winn MD, Parker PJ, Martin-Fernandez ML*.
Mol Cell Biol. 2011 Jun;31(11):2241-52
Monocyclic β-Lactams Are Selective, Mechanism-Based Inhibitors of Rhomboid Intramembrane Proteases
Pierrat OA*, Strisovsky K, Christova Y, Large J, Ansell K, Bouloc N, Smiljanic E, Freeman M.
ACS Chem Biol. 2011 Apr 15;6(4):325-35
Cy3 and Cy5 labelling of mouse EGF (Epidermal Growth Factor)
Single-Molecule Imaging and Fluorescence Lifetime Imaging Microscopy Show Different Structures for High- and Low-Affinity Epidermal Growth Factor Receptors in A431 Cells
Stephen E.D. Webb, Selene K. Roberts*, Sarah R. Needham*, Christopher J. Tynan, Daniel J. Rolfe, Martyn D. Winn, David T. Clarke, Roger Barraclough, and Marisa L. Martin-Fernandez*.
Biophys J. 2008 February 1; 94(3): 803–819
CRB will be attending and exhibiting at the following conferences and seminars:
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Request a Quotation
Should you require a quotation for any of our custom services, we have a clear cut and easy system that enables you to do so.
At Cambridge Research Biochemicals we have a fast-track quotation system that tailors your requirements, gives you all the information you need and a specific price within 24 hours. You are also given a personal contact so that if you have any further questions you can directly contact one of our approachable representatives who has the technical knowledge to assist you.
1   Go to:
2   Click on the relevant tab and register your details.
3   Once issued with a password log on to our quotation system using your customer account log-in and a quotation will be issued within 24 hours.
Telephone: +44(0) 1642 567 180
Fax: +44(0) 1642 567 181
17-18 Belasis Court, Belasis Hall Technology Park, Billingham, Cleveland, TS23 4AZ United Kingdom.