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Quotient Alliance:
CRB has recently partnered with Quotient Bioresearch to offer a streamlined Tritium radiolabelled peptide service
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Quotient Bioresearch, a leading provider of early stage and specialist drug development services to pharmaceutical, biotechnology and medical device clients worldwide, has signed a strategic partnership agreement with Cambridge Research Biochemicals to provide a joint consultancy solution for tritium peptide labelling, offering significant cost and time savings to customers.
The partnership will see Quotient Bioresearch and Cambridge Research Biochemicals, the world's second oldest peptide manufacturer, work together to supply both tritium radiolabelled and cold peptides as a package to a high quality with reliable delivery in a single shipment.
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Radiolabelled Peptides
Cambridge Research Biochemicals (CRB) is the second oldest custom manufacturer of peptides in the world, with over 30 years of experience of providing high quality bespoke peptides.
Strategic Alliances
CRB is able to leverage this expertise in peptide synthesis, through unique collaborations with major radio-synthesis groups, to provide peptides and proteins labelled with tritium (3H), carbon-14 (14C) and iodine-125 (125I).
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Features of Radiolabelling Service
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Product shipped directly from the radiochemical laboratory to the customer |
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CRB liaises with shipping broker to ensure smooth and problem free delivery to the customer |
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Trititated Peptides are usually supplied in 10% aqueous ethanol to improve stability |
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Cold reference peptide is made as standard to determine specific activity. Cold peptide can be used as diluent to improve stability of hot peptide |
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Small organic molecules can also be prepared radiolabelled |
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Custom aliquotting upon request |
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Pure specifically tritiated peptides can be made for use in receptor studies, metabolic studies and pharmacological investigation.
Tritium reduction by catalytic exchange is the simplest and most economic route for labelling peptides that do not contain Cysteine residues.
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Analogues of the peptide containing one or more of Phe, Pro, Tyr, or Leu residues substituted with a double bond or halogen moiety are synthesised at CRB and sent to the radio-synthesis group where these precursor peptides are then reduced with Tritium to generate the desired tritium labelled peptide with a specific activity in the range of 10-100Ci/mmole
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Iodine-125 (125I) labelled Peptides and proteins
Iodine-125, with a half-life of 60 days, is a cost-effective choice for labelling of peptides and proteins, as the reaction is performed directly on the peptide or protein sample. CRB offers a fixed fee service for iodination (2 x 50 mCi[125I]) and HPLC purification. For peptides and proteins containing tyrosine residues, iodination is carried out with Na125I activated with chloramin-T, lactoperoxidase or iodogen. Alternatively, free amino functions of the protein can be labelled using 125I-Bolton-Hunter reagent.
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Carbon-14 (14C) labelled Peptides
Carbon-14 should only be considered after Tritium for two reasons: Carbon-14 can only be incorporated in to peptides via an expensive Carbon-14 labelled amino acid or by addition to the N-terminus of the peptide (e.g. 14C-Acetic acid to form 14C-Acetyl-peptide) and secondly due to the thousand-fold reduction in specific activity.
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Tritium labelling
The simplest route to tritium labelled peptides involves the catalytic hydrogenation or hydrogenolysis of a peptide analogue containing unsaturated or iodinated precursor amino acid derivatives.
These peptides are assembled, purified and characterised in the same way as other peptides and then treated with tritium gas to generate the tritium labelled peptide.
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125I labelling
Labelling with iodine-125 normally involves incorporation of iodine-125 into tyrosine or histidine residues within the peptide or protein. This is done by treatment of the sample with Na125I in the presence of an oxidising reagent to generate a source of electrophilic iodine. Usually, one or two iodine atoms are added depending on conditions used. For peptides and proteins lacking accessible tyrosine residues, free amines can be derivatised with Bolton-Hunter reagent prior to iodination.
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14C labelling
The simplest approach to 14C labelling involves acetylation of free amino groups in the peptide with 14C-acetic acid to provide peptides with a specific activity of up to 120mCi/mmol. Glycine also can be prepared with one or both carbon atoms labelled with carbon-14 leading to a maximum specific activity of 100-120mCi/mmole and incorporated during peptide assembly.
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Should you require a quotation from any of our custom services, we have a clear cut and easy system that enables you to do so. Follow these three simple steps:
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Simply click on the relevant tab and register your details. |
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Once issued with a password you can then log on to our quotation system using your customer account log-in and a quotation will be issued within 24 hours. |
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At Cambridge Research Biochemicals we have a fast-track quotation system that tailors your requirements, gives you all the information you need and a specific price within 24 hours. You are also given a personal contact so that if you have any further questions you can directly contact one of our approachable representatives who has the technical knowledge to assist you.
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T +44 (0)1642 567180
F +44 (0)1642 567181
E enquiries@crbdiscovery.com
17-18 Belasis Court, Belasis Hall Technology Park, Billingham, Cleveland, TS23 4AZ United Kingdom
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