CRB Discovery

Antibody Labelling and Validation

In this issue
Antibody Labelling: CRB has a broad range of custom labelling services
Fragmentation: Antibody fragmentation for specific labelling
Antibody Validation: Western Blot analysis service

Antibody Labelling

Cambridge Research Biochemicals, the specialist peptide and custom antibody manufacturer based in the North East of England, has announced a significant expansion to its dye labelling offering. The company has signed an extension to its Supply & Licence Agreement with Life Technologies – Molecular Probes. The deal extends CRB’s licence to offer dye-labelled primary antibodies as well as peptides.

Find out more

Dye Selector
Fab Fragmentation

Fab Fragmentation

Immunoglobulins (IgG, IgM) can be selectively cleaved by proteases to give the antibody binding fragments (Fab, F(ab’)2 or Fab’) and the class defining fragment (Fc). These can be separated by affinity chromatography using Protein A which binds the Fc fragment and intact IgG but not the Fab fragments.

Antibody fragments offer a number of potential advantages over the intact molecule including reduced non-specific binding, higher sensitivity and better tissue penetration for IHC applications. The purified antibody fragments can be labelled in the same way as the intact antibody.

To request Fab Fragmentation from CRB call us on +44 (0)1642 567180

Antibody Validation

Cambridge Research Biochemicals offers a fully flexible custom Western Blot analysis service to complement its custom generated Target Antibodies™.

Western Blot validation may be carried out on any CRB generated custom polyclonal or monoclonal antibody.

Find out more

For more information on these topics please visit the Target Antibodies page on our website
Antibody Validation

Customer References

Cy3 and Cy5 labelling of mouse EGF (Epidermal Growth Factor)

Single–Molecule Imaging and Fluorescence Lifetime Imaging Microscopy Show Different Structures for High– and Low–Affinity Epidermal Growth Factor Receptors in A431 Cells.

S. E. D. Webb, S. K. Roberts, S. R. Needham, C. J. Tynan, D. J. Rolfe, M. D. Winn, D. T. Clarke, R. Barraclough & M. L. Martin-Fernandez.
Science and Technology Facilities Council & University of Liverpool
Biophysical Journal 2008, 94, 803-819

Alexa 488, Alexa 547, Atto 647N labelling of mouse EGF and affibodies

Optics clustered to output unique solutions: A multi–laser facility for combined single molecule and ensemble microscopy.

D. T. Clarke, S. W. Botchway, B. C. Coles, S. R. Needham, S. K. Roberts, D. J. Rolfe, C. J. Tynan, A. D. Ward, S. E. D. Webb, R. Yadav, L. Zanetti–Domingues & M. L. Martin–Fernandeza.
Science and Technology Facilities Council
Rev. Sci. Instrum. 82, 093705 (2011)

Atto 488 and Alexa 546 labelling of mouse EGF (Epidermal Growth Factor)

Human-EGFR aligned on the plasma membrane adopts key features of Drosophila–EGFR asymmetry.

C. J. Tynan, S. K. Roberts, D. J. Rolfe, D. T. Clarke, H. H. Loeffler, J. Kästner, M. D. Winn, P. J. Parker & M. L. Martin–Fernandez.
Science and Technology Facilities Council, University of Stuttgart, King’s College London, Cancer Research UK
Mol. Cell. Biol. June 2011 vol. 31. no. 11 2241-2252

Cambridge Research Biochemicals are proud sponsors of the following Cambridge Cell Biology Seminars 2012:

Dr Andrew Peden

Cambridge Institude for Medical Research
6 February 2012
Sackler Lecture Theatre
Insights into post-Golgi trafficking from a SNARE specific siRNA screen

Professor Mikael Simons

Max Planck Institut fur Experimentelle Medizin
5 March 2012
Sackler Lecture Theatre
Mechanisms of myelination in the central nervous system

Professor Dick McIntosh

University of Colorado at Boulder
23 April 2012
Sackler Lecture Theatre
Microtubule depolymerization as a cellular machine and proteins that bind preferentially to bending oligomers of tubulin

Request a quotation

Should you require a quotation from any of our custom services, we have a clear cut and easy system that enables you to do so.

At Cambridge Research Biochemicals we have a fast-track quotation system that tailors your requirements, gives you all the information you need and a specific price within 24 hours. You are also given a personal contact so that if you have any further questions you can directly contact one of our approachable representatives who has the technical knowledge to assist you.

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Click on the relevant tab and register your details.

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Once issued with a password log on to our quotation system using your customer account log-in and a quotation will be issued within 24 hours.
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