Cambridge Research Biochemicals (CRB) has provided Monoclonal Antibody services through partnerships with Babraham Bioscience Technologies Ltd & Epitomics Inc for six years.

In 2011, CRB expanded its capacity for Monoclonal Antibody production through a partnership with local Biological and Biomedical Sciences School at the University of Durham. The agreement allows access to additional analysis of antibodies from FACs to affinity rates.

Monoclonals offer many advantages over traditional polyclonals:

• PNA binds to complementary DNA/RNA with higher affinity and greater specificity than conventional oligonucleotides
• PNA is more stable than DNA or RNA as they are resistant to nucleases and proteases
• PNA can be hydridized at lower salt concentrations than DNA or RNA which leads to higher stringency

Our Monoclonal Antibody service is a way of generating antibodies in perpetuity. Once successfully made these antibodies can be duplicated time and time again to infinity.

• Expert advice on the vital first step of antigen selection from either your target protein or peptide sequence
• Design of screening strategy to select for clones against specific PTMs or protein regions
• Choice of appropriate species

• Extended immunisations to achieve optimum titre prior to cloning
• Development of screening assay to identify best clones
• Multiple sub-cloning to capture stable cell lines
• Scalable production and purification capacity

• Regular progress updates
• Defined check points for project progression
• Written reports available at end of each phase
• Back-up freezing of sub-clones and final cell line

Cambridge Research Biochemicals has recently introduced an additional Monoclonal Antibody service of scale-up production and purification of Cell lines. CRB generated clones or Customer’s own hybridomas can be scaled up to produce large quantities (grams) of purified Monoclonal antibody once a trial small scale (10mg of mAb) purification is undertaken.

• Transferral of cells to serum-free media
• Cells are cultured in 1 Litre cultivation as an initial trial
• 10mg purified antibody produced after initial trial
• Purification by protein A/G chromatography
• Evaluation of cell-line productivity for scale-up production
• Rapid Delivery of 3-4 weeks

Should you require a quotation from any of our custom services, we have a clear cut and easy system that enables you to do so.

At Cambridge Research Biochemicals we have a fast-track quotation system that tailors your requirements, gives you all the information you need and a specific price within 24 hours. You are also given a personal contact so that if you have any further questions you can directly contact one of our approachable representatives who has the technical knowledge to assist you.