Flagellin is the main structural protein of the bacterial flagella and acts as both a virulence factor helping bacteria invade host cells and is also recognised by the host to induce a potent immune response. Flagellin 22 (flg22) is the stretch of flagellin amino acids most conserved across bacterial species and is the minimal ligand… Read more »
Posts By: Sarah Smith
Understanding viruses is a key area of research for public health. There is still a lot that is not understood about how retroviruses replicate and infect host cells, leaving gaps in our understanding which if filled may lead to new retroviral drugs for treating these viral infections. Kate Bishop and her team at the Francis… Read more »
Andrew McKenzie and his team from the MRC Laboratory of Molecular Biology (LMB) enlisted the expertise of the team at Cambridge Research Biochemicals (CRB) to produce a highly specific antibody to the 2A peptide sequence. 2A was discovered in the foot-and-mouth disease virus (FMDV, a picornavirus) and is used in cloning vectors. The team at MRC… Read more »
The Covid-19 pandemic has caused widespread disruption around the world with around 195 million confirmed infections globally to date. Here at Cambridge Research Biochemicals (CRB) we have been supporting the phenomenal effort within the research community to characterise and study this new coronavirus as quickly and thoroughly as possible. We have added a range of… Read more »
The choice to use a peptide or a recombinant protein as the antigen to raise an antibody can be a difficult decision. Here we have outlined the main points to consider in making your choice easier.
Peptides can be used to make highly specific targeted antibodies.
Here at CRB we have an excellent success rate in raising antibodies to recognise PTMs, gained from our years of expertise and our dedication to each project ensuring the best possible outcomes.
Here at CRB we offer a wide range of hosts for antibody production, and whereas mammalian are often the preferred choice, chickens are also growing in popularity. The production of antibodies in the avian host has several advantages and can be an excellent choice in the right circumstances.
Here we outline some of the main advantages of raising antibodies in chicken.
Alain Kohl’s team at the MRC-University of Glasgow Centre for Virus Research chose to recruit the technical expertise of Cambridge Research Biochemicals (CRB) to raise custom polyclonal antibodies specific to the mosquito born Peribunyaviridae family virus; Cache Valley virus (CVV) as well as to Kairi-virus (KRIV). CVV has a tri-segmented, single-stranded genome with negative or ambi-sense polarity which encode… Read more »
Histone proteins help compact and organise the DNA of the nucleus, they also play key roles in orchestrating gene expression. The four core histones, H2A, H2B, H3 and H4 form a complex around which DNA is wound forming the nucleosome. Between the nucleosome is the internucleosomal DNA which is stabilised by the linker histone H1. Modifications of histones can alter the nature… Read more »
Histone proteins help compact and organise the DNA of the nucleus and provide it with structural support, they also play key roles in orchestrating gene expression. The four core histones, H2A, H2B, H3 and H4 form a complex comprising an H3-H4 tetramer and two H2A-H2B dimers, known as the histone octamer core. Around this core,… Read more »