Aurora™ Fluor 488 Anti-2SC antibody
Description
Application Data
Description
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Detects succinated cysteine residues on succinated proteins with high affinity. Conjugated to Aurora™ Fluor 488 fluorescent dye.
Application Data
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Catalogue number crb2105017 Antibody Aurora™ Fluor 488 Anti-2SC antibody Antigen Peptide KLH conjugated S-(2-succinyl)-cysteine Aliases S-(2-succinyl)-cysteine Host Species Anti-Rabbit Antibody Type Polyclonal Label Labelled with Aurora™ Fluor 488 under optimal conditions. Ex: 494nm Em:517nm Concentration 0.5mg/ml TEA Validation ELISA Target 2SC Storage Stabilisers Supplied in PBS containing 0.01% sodium azide. The product should be stored undiluted and protected from prolonged exposure to light. Short term storage between 2-8°C and long term storage below -20°C Disease Area Cancer Specificity Succinated cysteine residues Post-translational Modification Succination Storage Supplied in PBS containing 0.01% sodium azide. The product should be stored undiluted and protected from prolonged exposure to light. Short term storage between 2-8°C and long term storage below -20°C Citations Burgener et al., (2019). SDHA gain-of-function engages inflammatory mitochondrial retrograde signaling via KEAP1-Nrf2. Nat Immunol. 20(10):1311-1321. PMID: 31527833
Casey et al., (2020). Fumarate Metabolic Signature for the Detection of Reed Syndrome in Humans.Clin Cancer Res. 26(2):391-396. PMID: 31636096. doi: 10.1158/1078-0432.
Hum Pathol. PMID: 32681944. doi: 10.1016/j.humpath.2020.07.014.
Secondary Renal Neoplasia Following Chemotherapy or Radiation in Pediatric Patients.Äyräväinen et al., (2020). Systematic molecular and clinical analysis of uterine leiomyomas from fertile-aged women undergoing myomectomy. Human Reproduction. deaa187, https://doi.org/10.1093/humrep/deaa187
Kiyozawa et al., (2022) Approach for reclassification of collecting duct carcinoma and comparative histopathological analysis with SMARCB1/INI1-deficient renal cell carcinoma and fumarate hydratase-deficient renal cell carcinoma. Hum Pathol. https://doi.org/10.1016/j.humpath.2022.03.002
References Alderson, N., Wang, Y., Blatnik, M., Frizzell, N., Walla, M., Lyons, T., Alt, N., Carson, J., Nagai, R., Thorpe, S. and Baynes, J. (2006). S-(2-Succinyl)cysteine: A novel chemical modification of tissue proteins by a Krebs cycle intermediate. Arch Biochem Biophys, 450(1), 1-8. PMID: 16624247
Nagai, R., Brock, J., Blatnik, M., Baatz, J., Bethard, J., Walla, M., Thorpe, S., Baynes, J. and Frizzell, N. (2007). Succination of Protein Thiols during Adipocyte Maturation: a biomarker of mitochondrial stress. J Biol Chem, 282(47), 34219-34228. PMID: 17726021
Merkley, E., Metz, T., Smith, R., Baynes, J. and Frizzell, N. (2013). The succinated proteome. Mass Spectrom Rev, 33(2), 98-109. PMID: 24115015
Succination is a stable post-translational modification of cysteine residues, which modifies protein function or turnover in response to a changing intracellular redox environment. Succination occurs when the Krebs cycle intermediate, fumarate, reacts with cysteine yielding S-(2-succino)cysteine (2SC).
A wide range of proteins are subject to succination, including enzymes, adipokines, cytoskeletal proteins and ER chaperones and succination has been shown to have roles in regulatory biology.
An increase in succination of adipocyte proteins is seen in diabetes mellitus and results from nutrient excess derived mitochondrial stress. 2SC therefore serves as a biomarker of mitochondrial stress or dysfunction in chronic diseases, such as obesity, diabetes, and cancer.
This Antibody is conjugated to a popular bright green flourescent dye: Aurora™ Fluor 488.