Aurora™ Fluor 488 Anti-2SC antibody 50ug

£500.00
  • Description

  • Application Data

Description

Detects succinated cysteine residues on succinated proteins with high affinity. Conjugated to Aurora™ Fluor 488 fluorescent dye.

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Application Data

Catalogue number crb2105017e/6773
Antibody Aurora™ Fluor 488 Anti-2SC antibody 50ug
Antigen Peptide KLH conjugated S-(2-succinyl)-cysteine
Aliases S-(2-succinyl)-cysteine
Host Species Anti-Rabbit
Antibody Type Polyclonal
Label Labelled with Aurora™ Fluor 488 under optimal conditions. Ex: 494nm Em:517nm
Concentration 0.5mg/ml TEA
Validation ELISA
Target 2SC
Storage Stabilisers Supplied in PBS containing 0.01% sodium azide. The product should be stored undiluted and protected from prolonged exposure to light. Short term storage between 2-8°C and long term storage below -20°C
Disease Area Cancer
Specificity Succinated cysteine residues
Post-translational Modification Succination
Storage Supplied in PBS containing 0.01% sodium azide. The product should be stored undiluted and protected from prolonged exposure to light. Short term storage between 2-8°C and long term storage below -20°C
Citations

Burgener et al., (2019). SDHA gain-of-function engages inflammatory mitochondrial retrograde signaling via KEAP1-Nrf2. Nat Immunol. 20(10):1311-1321. PMID: 31527833

Casey et al., (2020). Fumarate Metabolic Signature for the Detection of Reed Syndrome in Humans.Clin Cancer Res.  26(2):391-396. PMID: 31636096. doi: 10.1158/1078-0432.

Gupta et al., (2020). Secondary Renal Neoplasia Following Chemotherapy or Radiation in Pediatric Patients. Hum Pathol PMID: 32681944. doi: 10.1016/j.humpath.2020.07.014.

Äyräväinen et al., (2020). Systematic molecular and clinical analysis of uterine leiomyomas from fertile-aged women undergoing myomectomy. Human Reproduction. deaa187, https://doi.org/10.1093/humrep/deaa187

Kiyozawa  et al., (2022) Approach for reclassification of collecting duct carcinoma and comparative histopathological analysis with SMARCB1/INI1-deficient renal cell carcinoma and fumarate hydratase-deficient renal cell carcinoma. Hum Pathol. https://doi.org/10.1016/j.humpath.2022.03.002

References

Alderson, N., Wang, Y., Blatnik, M., Frizzell, N., Walla, M., Lyons, T., Alt, N., Carson, J., Nagai, R., Thorpe, S. and Baynes, J. (2006). S-(2-Succinyl)cysteine: A novel chemical modification of tissue proteins by a Krebs cycle intermediate. Arch Biochem Biophys, 450(1), 1-8. PMID: 16624247

 

Nagai, R., Brock, J., Blatnik, M., Baatz, J., Bethard, J., Walla, M., Thorpe, S., Baynes, J. and Frizzell, N. (2007). Succination of Protein Thiols during Adipocyte Maturation: a biomarker of mitochondrial stress. J Biol Chem, 282(47), 34219-34228. PMID: 17726021

 

Merkley, E., Metz, T., Smith, R., Baynes, J. and Frizzell, N. (2013). The succinated proteome. Mass Spectrom Rev, 33(2), 98-109. PMID: 24115015

Material Safety Data Sheet (MSDS)

Succination is a stable post-translational modification of cysteine residues, which modifies protein function or turnover in response to a changing intracellular redox environment. Succination occurs when the Krebs cycle intermediate, fumarate, reacts with cysteine yielding S-(2-succino)cysteine (2SC).

A wide range of proteins are subject to succination, including enzymes, adipokines, cytoskeletal proteins and ER chaperones and succination has been shown to have roles in regulatory biology.

An increase in succination of adipocyte proteins is seen in diabetes mellitus and results from nutrient excess derived mitochondrial stress. 2SC therefore serves as a biomarker of mitochondrial stress or dysfunction in chronic diseases, such as obesity, diabetes, and cancer.

This Antibody is conjugated to a popular bright green flourescent dye: Aurora™ Fluor 488.

Aurora™ Fluor 488 Anti-2SC antibody 50ug

50μg
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