Alexa Fluor® 488 Anti-Phospho-CDK2 (pY15) antibody
Description
Application Data
Description
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Recognises CDK2 phosphorylated on tyrosine 15. Conjugated to Alexa Fluor® 488 fluorescent dye
Application Data
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Data Sheet Material Safety Data Sheet (MSDS)Catalogue number crb2115014e Antibody Alexa Fluor® 488 Anti-Phospho-CDK2 (pY15) antibody Antigen Peptide KLH conjugated synthetic peptide crb1200296 Protein ID UnitProtKB - P24941 Aliases Cyclin-dependent kinase 2, CDK2, CDK-2, Cell division protein kinase 2, p33 protein kinase Cross-Reactivity Human, rat, mouse Target Protein Species Human, rat, mouse Host Species Anti-Rabbit Antibody Type Polyclonal Concentration 1mg/ml Validation ELISA Target phosphorylated CDK2 (pY15) Storage Stabilisers This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4°C for short term storage and -20°C for long term storage. Avoid repeated freeze/ thaw cycles. Family Ser/Thr protein kinases (cyclin-dependent) Disease Area Cancer Specificity Protein Storage This material is supplied in PBS containing 0.01% sodium azide and 1% trehalose. The product should be stored at +4°C for short term storage and -20°C for long term storage. Avoid repeated freeze/ thaw cycles. Citations Hughes, B., Sidorova, J., Swanger, J., Monnat, R. and Clurman, B. (2013). Essential role for Cdk2 inhibitory phosphorylation during replication stress revealed by a human Cdk2 knockin mutation. PNAS, 110(22), 8954-8959. PMID: 23671119
Ji, X., Humenik, J., Yang, D. and Liebhaber, S. (2017). PolyC-binding proteins enhance expression of the CDK2 cell cycle regulatory protein via alternative splicing. Nucleic Acids Res, 46(4), 2030-2044. PMID: 29253178
References Hughes, B., Sidorova, J., Swanger, J., Monnat, R. and Clurman, B. (2013). Essential role for Cdk2 inhibitory phosphorylation during replication stress revealed by a human Cdk2 knockin mutation. PNAS, 110(22), 8954-8959. PMID: 23671119
Ji, X., Humenik, J., Yang, D. and Liebhaber, S. (2017). PolyC-binding proteins enhance expression of the CDK2 cell cycle regulatory protein via alternative splicing. Nucleic Acids Res, 46(4), 2030-2044. PMID: 29253178
Cyclin-dependent kinases (CDKs) are regulated by positive and negative phosphorylation. T14/Y15 phosphorylation by the WEE1 and MYT1 kinases inhibits CDKs by preventing ATP binding, and these conserved phosphorylations are highly regulated during the cell cycle and by signalling pathways. WEE1 and MYT1 are opposed by cell division cycle 25 (CDC25) phosphatases, which, in mammals, comprise three related enzymes that dephosphorylate T14/Y15 to promote CDK activity.
Cdk2 inhibition is involved in DNA replication and S-phase DNA damage checkpoints. CDK2 T14/Y15 phosphorylation is not required for DNA damage-induced cell cycle arrest, but instead has roles in S-phase entry and centrosome duplication.
This antibody is conjugated to Alexa Fluor® 488. Alexa Fluor® 488 is a popular bright green fluorescent dye with high pH-stability.