Alexa Fluor® 488 Anti-NR1 4b antibody
Description
Application Data
Description
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Recognises the 4b splice variant of the NMDA receptor subunit NR1. Conjugated to Alexa Fluor® 488 fluorescent dye
Application Data
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Data Sheet Material Safety Data Sheet (MSDS)Catalogue number crb2115008e Antibody Alexa Fluor® 488 Anti-NR1 4b antibody Antigen Peptide KLH conjugated synthetic peptide crb1200277e Protein ID UniProtKB - Q05586 Aliases NR1, MRD8, NMDAR1, NMDA Zeta-1, NMD-R1, GluN1, glutamate ionotropic receptor NMDA type subunit 1, NMDA NR1 Subunit Cross-Reactivity Human, Rat, Mouse Target Protein Species Human, Rat, Mouse Host Species Anti-Rabbit Antibody Type Polyclonal Label Labelled with Alexa Fluor® 488 under optimal conditions. Ex: 495nm Em:519nm Concentration 0.64mg/ml Validation ELISA Target NR1 4b Storage Stabilisers Supplied in PBS containing 0.01% sodium azide. The product should be stored undiluted and protected from prolonged exposure to light. Short term storage between 2-8°C and long term storage below -20°C Family Glutamate-gated ion channel family. Disease Area Neurodegenerative diseases Specificity NR1 Splice variant 4b Storage Supplied in PBS containing 0.01% sodium azide. The product should be stored undiluted and protected from prolonged exposure to light. Short term storage between 2-8°C and long term storage below -20°C Citations Donaldson, L. and Beazley-Long, N. (2016). Alternative RNA splicing: contribution to pain and potential therapeutic strategy. Drug Discov Today, 21(11), 1787-1798. PMID: 27329269
Ewald, R. (2009). Cloning and phylogenetic analysis of NMDA receptor subunits NR1, NR2A and NR2B in Xenopus laevis tadpoles. Front Mol Neurosci, 2. PMID: 19826620
References Donaldson, L. and Beazley-Long, N. (2016). Alternative RNA splicing: contribution to pain and potential therapeutic strategy. Drug Discov Today, 21(11), 1787-1798. PMID: 27329269
Ewald, R. (2009). Cloning and phylogenetic analysis of NMDA receptor subunits NR1, NR2A and NR2B in Xenopus laevis tadpoles. Front Mol Neurosci, 2. PMID: 19826620
N-methyl-D-aspartate receptors (NMDARs) are ligand-gated ionotropic glutamate receptors that mediate excitatory synaptic transmission and play important roles in many aspects of nervous system function including: synaptic plasticity; learning and memory; neuronal development and circuit formation. NMDARs have been implicated in various neuronal disorders. NMDARs are heteromers consisting of an obligate NR1 subunit and most commonly one or two kinds of NR2 subunits or occasionally NR3 subunits.
The NR1 subunit is alternatively spliced yielding eight possible isoforms characterised by the inclusion or deletion of exons 5, 21 and 22. The splice variants are developmentally and spatially regulated and modulate the biophysical properties of NMDARs and alter the trafficking behaviour of NR1.
NR1-4b Splice variants includes exons 5, 21, and 22 and is expressed in the dorsal horn of the spinal cord.The involvement of NMDAR in the central nervous system (CNS) has become a focus area for neurodegenerative diseases such as Alzheimer’s disease, epilepsy and ischemic neuronal cell death.
This antibody is conjugated to Alexa Fluor® 488. Alexa Fluor® 488 is a popular bright green fluorescent dye with high pH-stability.