Alexa Fluor® 488 Anti-HAND-1 antibody
Description
Application Data
Description
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Recognises HAND1, a twist family bHLH transcription factor essential for several developmental processes. Conjugated to AF488, a bright green fluorescent dye
Application Data
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Data Sheet Material Safety Data Sheet (MSDS)Catalogue number crb2115015e Antibody Alexa Fluor® 488 Anti-HAND-1 antibody Antigen Peptide KLH conjugated synthetic peptide crb1200298f Protein ID UniProtKB - O96004 Cross-Reactivity Human, Rat, Mouse Target Protein Species Human, Rat, Mouse Host Species Anti-Sheep Antibody Type Polyclonal Concentration 0.69mg/ml Validation ELISA Target HAND-1 Storage Stabilisers Supplied in PBS containing 0.01% sodium azide. The product should be stored undiluted and protected from prolonged exposure to light. Short term storage between 2-8°C and long term storage below -20°C. Family Basic helix-loop-helix Disease Area Cardiovascular Specificity Protein Storage Supplied in PBS containing 0.01% sodium azide. The product should be stored undiluted and protected from prolonged exposure to light. Short term storage between 2-8°C and long term storage below -20°C. Citations Barnes, R. and Firulli, A. (2009). A twist of insight - the role of Twist-family bHLH factors in development. Int J Dev Biol, 53(7), 909-924. PMID: 19378251
Firulli, B., Fuchs, R., Vincentz, J., Clouthier, D. and Firulli, A. (2014). Hand1 phosphoregulation within the distal arch neural crest is essential for craniofacial morphogenesis. Development, 141(15), 3050-3061. PMID: 25053435
References Barnes, R. and Firulli, A. (2009). A twist of insight – the role of Twist-family bHLH factors in development. Int J Dev Biol, 53(7), 909-924. PMID: 19378251
Firulli, B., Fuchs, R., Vincentz, J., Clouthier, D. and Firulli, A. (2014). Hand1 phosphoregulation within the distal arch neural crest is essential for craniofacial morphogenesis. Development, 141(15), 3050-3061. PMID: 25053435
Heart and neural crest derivatives expressed 1 (HAND1) is a basic helix-loop-helix (bHLH) transcription factor belonging to the twist family. Twist-family transcription factors play essential roles in a number of developmental programs and function by dimerising with other bHLH members and binding to cis- regulatory elements, called E-boxes. HAND1 can form homodimers as well as interact with other tissue restricted bHLH proteins, such as HAND2. Dimer partner choice infers differential regulation on twist family proteins and is regulated by phosphorylation. Protein Kinase A and C (PKA and PKC) can phosphorylate HAND1, while β56δ-containing protein phosphatase 2A (PP2A) can dephosphorylate HAND1.
HAND1 is required for proper cardiac morphogenesis and is essential for extra-embryonic and trophoblast cell differentiation. Hand1 is expressed in several foetal tissues and in adult heart tissue where it may have a role in preventing hypertrophy.
This antibody has Alexa Fluor® 488 conjugated to it. Alexa Fluor® 488 is a popular bright green fluorescent dye with high PH-stability.