Alexa Fluor® 488 Anti-Cyclin D1 antibody
Description
Application Data
Description
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Recognises Cyclin D1, a key regulator of cell proliferation and tumourigenesis. Antibody is conjugated to Alexa Fluor® 488 fluorescent dye
Application Data
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Data Sheet Material Safety Data Sheet (MSDS)Catalogue number crb2115024e Antibody Alexa Fluor® 488 Anti-Cyclin D1 antibody Antigen Peptide KLH conjugated synthetic peptide crb1200305e Protein ID UnitProtKB - P24385 Aliases CCND1 Cross-Reactivity Human Target Protein Species Human Host Species Anti-Rabbit Antibody Type Polyclonal Label Labelled with Alexa Fluor® 488 under optimal conditions. Ex: 495nm Em:519nm Concentration 0.53mg/ml Validation ELISA Target Cyclin D1 Storage Stabilisers Supplied in PBS containing 0.01% sodium azide. The product should be stored undiluted and protected from prolonged exposure to light. Short term storage between 2-8°C and long term storage below -20°C Family Cyclin Disease Area Cancer, cell cycle Specificity Protein Storage Supplied in PBS containing 0.01% sodium azide. The product should be stored undiluted and protected from prolonged exposure to light. Short term storage between 2-8°C and long term storage below -20°C Citations Pysz, M., Hao, F., Hizli, A., Lum, M., Swetzig, W., Black, A. and Black, J. (2014). Differential Regulation of Cyclin D1 Expression by Protein Kinase C α and ϵ Signaling in Intestinal Epithelial Cells. J Biol Chem, 289(32), 22268-22283. PMID: 24914206
Qie, S. and Diehl, J. (2016). Cyclin D1, cancer progression, and opportunities in cancer treatment. J Mol Med, 94(12), 1313-1326. PMID: 27695879
References Pysz, M., Hao, F., Hizli, A., Lum, M., Swetzig, W., Black, A. and Black, J. (2014). Differential Regulation of Cyclin D1 Expression by Protein Kinase C α and ϵ Signaling in Intestinal Epithelial Cells. J Biol Chem, 289(32), 22268-22283. PMID: 24914206
Qie, S. and Diehl, J. (2016). Cyclin D1, cancer progression, and opportunities in cancer treatment. J Mol Med, 94(12), 1313-1326. PMID: 27695879
Cyclin D1 is a key regulator of cell proliferation and its expression and accumulation within cells is under tight control. Cyclin D1 is the regulatory subunit of cyclin-dependent kinases 4 and 6. These activated cyclin dependent kinases then phosphorylate the retinoblastoma protein (Rb) and drive G1 to S phase progression. Cyclin D1 promotes cell proliferation through interaction with transcription factors such as the oestrogen receptor and specificity protein 1 (Sp1). Cell proliferation is extremely sensitive to altered levels of cyclin D1, with even modest changes in its expression having noticeable effects on cell cycle progression. Cyclin D1 is a proto-oncogene and its overexpression is one of the most frequent alterations seen in multiple cancer types.
This antibody is conjugated to Alexa Fluor® 488. Alexa Fluor® 488 is a popular bright green fluorescent dye with high pH-stability.