Ubiquitin K63 Heavy
TLSDYNIQ-[K(GG)]-ESTLHLVL-[U-13C6,15N4-Arg]-acid
Description
Application Data
Description
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Sequence corresponding to the bond between Lys63 side-chain linked mammalian ubiquitin proteins. Contains arginine residue labelled with 13C and 15N isotopes
Application Data
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Data Sheet Material Safety Data Sheet (MSDS)Catalogue number crb1300818 Molecular Weight 2253.2 Sequence (one letter code) TLSDYNIQ-[K(GG)]-ESTLHLVL-[U-13C6,15N4-Arg]-acid
Sequence (three letter code) H-Thr-Leu-Ser-Asp-Tyr-Asn-Ile-Gln-[Lys-(Gly-Gly)]-Glu-Ser-Thr-Leu-His-Leu-Val-Leu-[U-13C6,15N4-Arg]-acid
Purity >95% Storage -20°C Citations Heunis et al., (2020). Technical report: Targeted proteomic analysis reveals enrichment of atypical ubiquitin chains in contractile murine tissues. Journ. Prot. Res., 229: 103963. doi: 10.1016/j.jprot.2020.103963.
References Ohtake et al (2018) K63 ubiquitylation triggers proteasomal degradation by seeding branched ubiquitin chains. PNAS 115(7) E1401 PMID: 29378950
Swatek and Komander (2016) Ubiquitin modifications. Cell Res. 26(4) 399 PMID: 27012465
Holzer et al (2012) Neuropeptide Y, peptide YY and pancreatic polypeptide in the gut–brain axis. Neuropeptides 46(6) 261 PMID: 22979996
Manufactured in: United Kingdom This sequence corresponds to the peptide bond between mammalian Lys63- (K63) linked Ub proteins; where (GG) corresponds to the C-terminus of the side chain appended Ub.
K63-linked chains are the second most abundant linkage types found in cells and are modified with Met1-linked chains in mixed or branched architectures. K63-linked chains have a variety of non-degradative roles including; roles in intracellular trafficking, kinase signalling, DNA damage response, inflammatory signalling and NF-κB activation, however they may also be involved in some proteosome dependent pathways
The N-terminal arginine residue of this peptide is isotopically labelled with carbon-13 (6) and nitrogen-15 (4), giving this peptide a mass increase of 10 compared to the unlabelled peptide.
Ubiquitin K63 Heavy
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