Ubiquitin K29 Heavy
Sequence corresponding to the bond between Lys29 side-chain linked mammalian ubiquitin proteins. Contains lysine residue labelled with 13C and 15N isotopes
Catalogue number crb1300812 Molecular Weight 823.5 Sequence (one letter code)
Sequence (three letter code)
Purity >95% Storage -20°C Citations
Heunis et al., (2020). Technical report: Targeted proteomic analysis reveals enrichment of atypical ubiquitin chains in contractile murine tissues. Journ. Prot. Res., 229: 103963. doi: 10.1016/j.jprot.2020.103963.
Swatek, K. and Komander, D. (2016). Ubiquitin modifications. Cell Res, 26(4), 399-422. PMID: 27012465
Manufactured in: United Kingdom
This sequence corresponds to the peptide bond between mammalian Lys29- (K29) linked Ub proteins; where (GG) corresponds to the C-terminus of the side chain appended Ub.
K29-linked chains are assembled by the HECT E3 ligase KIAA10/UBE3C that assembles K29- and K48-linked polyubiquitin chains. K29-linked polyUbs are involved in growth and development-associated pathways (Wnt/β-catenin signalling), and are also implicated in regulation of mRNA stability and the epigenetic regulation and transcription of a set of interleukins (IL12 and IL23). In addition to this, K29-linked chains are also associated with the 26S proteasome, which suggests a role as a proteasomal degradation signal.
The C-terminal lysine residue of this peptide is isotopically labelled with carbon-13 (6) and nitrogen-15 (2), giving this peptide a mass increase of 8 compared to the unlabelled peptide.