Thyroglobulin (Tg-VIF) Heavy
VIFDANAPVAV-[U-13C6, U-15N4-Arg]-acid
Description
Application Data
Description
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Thyroglobulin (Tg-VIF) Heavy
Application Data
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Data Sheet Material Safety Data Sheet (MSDS)Catalogue number crb1301707 Molecular Weight 1280.7 Sequence (one letter code) VIFDANAPVAV-[U-13C6, U-15N4-Arg]-acid
Sequence (three letter code) H-Val-Ile-Phe-Asp-Ala-Asn-Ala-Pro-Val-Ala-Val-[U-13C6, U-15N4-Arg]-OH
Purity >95% Storage -20°C References Kushnir et al., (2013). Measurement of thyroglobulin by liquid chromatography-tandem mass spectrometry in serum and plasma in the presence of antithyroglobulin autoantibodies. Clin Chem., 59(6): 982. doi: 10.1373/clinchem.2012.195594.
Nishihara et al., (2022). Serum thyroglobulin evaluation on LC-MS/MS and immunoassay in TgAb-positive patients with papillary thyroid carcinoma. Eur. Thyroid J., 11: 1. Doi: 10.1530/ETJ-21-0041.
Serum levels of thyroglobulin (Tg) is considered a valuable measurement for quantitively evaluating thyroid cancer treatment efficacy. Immunoassays are commonly used for Tg level evaluation but return many false negatives. This is due to a large proportion of patients expressing anti-Tg antibodies (TgAb), which outcompete the reagent antibodies for Tg.
Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is potentially better at quantification of Tg as TgAb should less influence it.
The Tg-VIF heavy arginine residue at position 12 is isotopically labelled with carbon-13 and nitrogen-15. This will allow absolute quantification applications such as quantitative proteomics, the quantification of complex protein mixtures at very low concentrations, or NMR studies.