Thyroglobulin (Tg-FSP)
FSPDDSAGASALLR-acid
Description
Application Data
Description
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Tg-FSP is a doubly charged peptide precursor ion of the FSP peptide attached to thyroglobulin (Tg). This tryptic Tg-FSP peptide is easily detectable and quantified by liquid chromatography-tandem mass spectrometry (LC-MS/MS).
Application Data
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Data Sheet Data Sheet Material Safety Data Sheet (MSDS)Catalogue number crb1001701 Molecular Weight 1405.7 Sequence (one letter code) FSPDDSAGASALLR-acid
Sequence (three letter code) H-Phe-Ser-Pro-Asp-Asp-Ser-Ala-Gly-Ala-Ser-Ala-Leu-Leu-Arg-OH
Purity >95% References Shi et al., (2022). A distributable LC-MS/MS method for the measurement of serum thyroglobulin. JMSACL, 26: 28. doi: 10.1016/j.jmsacl.2022.09.005
Manufactured in: United Kingdom Thyroglobulin (Tg) is a widely used biomarker of various differentiated thyroid cancer (DTC); Tg is a substrate for thyroid hormone production. Detection and quantification of serum thyroglobulin levels remain challenging due to Tg’s size, heterogeneity, and thyroglobulin autoantibodies (TgAb). Immunoassays offer the opportunity to tailor DTC treatments, but many patients are TgAb positive, excluding them from analysis during regression.
Liquid chromatography-tandem mass spectrometry (LC-MS/MS) can overcome immunoassay issues by digestion of Tg to a tryptic peptide removing the interference from TgAbs. The addition of a doubly charged peptide precursor ion, FSP peptide, allows easy detection of Tg-FSP by an anti-FSP antibody that is reliable and quantifiable.