[5-TAMRA]/[Lys(BHQ-2)] Ubiquitin

[5-TAMRA]-VLRLRGG-[Lys(BHQ-2)]-amide

  • Description

  • Application Data

Description

FRET peptide substrate for the severe acute respiratory syndrome coronavirus main protease (SARS-CoV Mpro). Fluorescence can be detected upon cleavage of the peptide by SARS-CoV Mpro

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Application Data

Catalogue number crb1101610
Molecular Weight 1812.9
Sequence (one letter code)

[5-TAMRA]-VLRLRGG-[Lys(BHQ-2)]-amide

Sequence (three letter code)

[5-TAMRA]-Val-Leu-Arg-Leu-Arg-Gly-Gly-[Lys(BHQ-2)]-NH2

Aliase Ubiquitin
Modifications Where BHQ-2 is Black Hole Quencher 2
Purity >95%
Storage -20°C
References

Yang et al (2005) Design of Wide-Spectrum Inhibitors Targeting Coronavirus Main Proteases. PLoS Biol. 3(10) e324 www.ncbi.nlm.nih.gov/pubmed/16128623   

 

Zhao et al (2008). Structure of the Main Protease from a Global Infectious Human Coronavirus, HCoV-HKU1. J. Virol. 82(17) 8647 www.ncbi.nlm.nih.gov/pubmed/18562531

Manufactured in: United Kingdom
Material Safety Data Sheet (MSDS)

This peptide contains an N-terminal a 5-carboxytetramethylrhodamine (5-TAMRA), a widely used fluorescent dye which excites at 546 nm and emits at 579 nm and a black hole quencher 2 (BHQ-2) group.

The fluorescence from 5-TAMRA is efficiently quenched by resonance energy transfer to the BHQ-2 group when the peptide is intact, however upon cleavage of the peptide by Mpro, 5-TAMRA and BHQ-2 are separated, allowing fluorescence to be detected. This therefore represents a useful tool for investigating Mpro activity.

[5-TAMRA]/[Lys(BHQ-2)] Ubiquitin

Cat No.Pack SizePriceQty.
0.5mg£200.00
1mg£300.00
5mg£1,200.00
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