[5-TAMRA]/[Lys(BHQ-2)] Ubiquitin
[5-TAMRA]-VLRLRGG-[Lys(BHQ-2)]-amide
Description
Application Data
Description
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FRET peptide substrate for the severe acute respiratory syndrome coronavirus main protease (SARS-CoV Mpro). Fluorescence can be detected upon cleavage of the peptide by SARS-CoV Mpro
Application Data
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Material Safety Data Sheet (MSDS)Catalogue number crb1101610 Molecular Weight 1812.9 Sequence (one letter code) [5-TAMRA]-VLRLRGG-[Lys(BHQ-2)]-amide
Sequence (three letter code) [5-TAMRA]-Val-Leu-Arg-Leu-Arg-Gly-Gly-[Lys(BHQ-2)]-NH2
Aliase Ubiquitin Modifications Where BHQ-2 is Black Hole Quencher 2 Purity >95% Storage -20°C References Yang et al (2005) Design of Wide-Spectrum Inhibitors Targeting Coronavirus Main Proteases. PLoS Biol. 3(10) e324 www.ncbi.nlm.nih.gov/pubmed/16128623
Zhao et al (2008). Structure of the Main Protease from a Global Infectious Human Coronavirus, HCoV-HKU1. J. Virol. 82(17) 8647 www.ncbi.nlm.nih.gov/pubmed/18562531
Manufactured in: United Kingdom This peptide contains an N-terminal a 5-carboxytetramethylrhodamine (5-TAMRA), a widely used fluorescent dye which excites at 546 nm and emits at 579 nm and a black hole quencher 2 (BHQ-2) group.
The fluorescence from 5-TAMRA is efficiently quenched by resonance energy transfer to the BHQ-2 group when the peptide is intact, however upon cleavage of the peptide by Mpro, 5-TAMRA and BHQ-2 are separated, allowing fluorescence to be detected. This therefore represents a useful tool for investigating Mpro activity.