[5-TAMRA]/[Lys(BHQ-2)]-CoV Main Protease (Mpro) Substrate


  • Description

  • Application Data


FRET peptide substrate for the severe acute respiratory syndrome coronavirus main protease (SARS-CoV Mpro). Fluorescence can be detected upon cleavage of the peptide by SARS-CoV Mpro

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Application Data

Catalogue number crb1101530
Molecular Weight 2048.0
Sequence (one letter code)


Sequence (three letter code)


Aliase Coronavirus, SARS-CoV-2
Modifications Where BHQ-2 is Black Hole Quencher 2
Purity >95%
Storage -20°C

Redhead et al (2021) Bispecific repurposed medicines targeting the viral and immunological arms of COVID-19. Sci Rep. 2021 11(1):13208. PMID: 34168183


Yang et al (2005) Design of Wide-Spectrum Inhibitors Targeting Coronavirus Main Proteases. PLoS Biol. 3(10) e324 www.ncbi.nlm.nih.gov/pubmed/16128623


Zhao et al (2008). Structure of the Main Protease from a Global Infectious Human Coronavirus, HCoV-HKU1. J. Virol. 82(17) 8647 www.ncbi.nlm.nih.gov/pubmed/18562531

Manufactured in: United Kingdom
Data Sheet Material Safety Data Sheet (MSDS)

Fluorescently labelled substrate for the severe acute respiratory syndrome coronavirus main protease (SARS-CoV Mpro). The substrate sequence is derived from residues P4–P5′ of the SARS-CoV Mpro N-terminal autoprocessing site which has the sequence AVLQSGFRK. SARS-CoV Mpro is a key antiviral target.

This peptide contains an N-terminal a 5-carboxytetramethylrhodamine (5-TAMRA), a widely used fluorescent dye which excites at 546 nm and emits at 579 nm and a black hole quencher 2 (BHQ-2) group. This Mpro substrate is used in Mpro inhibition assays. The compound being tested for its inhibitory capacity of Mpro is added alongside [5-TAMRA]/[Lys(BHQ-2)]-CoV, after a set time period, the amount of fluorescence released is read as a percentage inhibition by a plate reader.

The fluorescence from 5-TAMRA is efficiently quenched by resonance energy transfer to the BHQ-2 group when the peptide is intact, however upon cleavage of the peptide by Mpro, 5-TAMRA and BHQ-2 are separated, allowing fluorescence to be detected. This therefore represents a useful tool for investigating Mpro activity.

[5-TAMRA]/[Lys(BHQ-2)]-CoV Main Protease (Mpro) Substrate

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