[5-TAMRA]/[Lys(BHQ-2)]-CoV Main Protease (Mpro) Substrate
[5-TAMRA]-AVLQSGFR-[Lys(BHQ-2)]-K-amide
Description
Application Data
Description
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FRET peptide substrate for the severe acute respiratory syndrome coronavirus main protease (SARS-CoV Mpro). Fluorescence can be detected upon cleavage of the peptide by SARS-CoV Mpro
Application Data
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Data Sheet Material Safety Data Sheet (MSDS)Catalogue number crb1101530 Molecular Weight 2048.0 Sequence (one letter code) [5-TAMRA]-AVLQSGFR-[Lys(BHQ-2)]-K-amide
Sequence (three letter code) [5-TAMRA]-Ala-Val-Leu-Gln-Ser-Gly-Phe-Arg-Lys(BHQ-2)-Lys-NH2
Aliase Coronavirus, SARS-CoV-2 Modifications Where BHQ-2 is Black Hole Quencher 2 Purity >95% Storage -20°C Citations Redhead et al (2021) Bispecific repurposed medicines targeting the viral and immunological arms of COVID-19. Sci Rep. 2021 11(1):13208. PMID: 34168183
References Yang et al (2005) Design of Wide-Spectrum Inhibitors Targeting Coronavirus Main Proteases. PLoS Biol. 3(10) e324 www.ncbi.nlm.nih.gov/pubmed/16128623
Zhao et al (2008). Structure of the Main Protease from a Global Infectious Human Coronavirus, HCoV-HKU1. J. Virol. 82(17) 8647 www.ncbi.nlm.nih.gov/pubmed/18562531
Manufactured in: United Kingdom Fluorescently labelled substrate for the severe acute respiratory syndrome coronavirus main protease (SARS-CoV Mpro). The substrate sequence is derived from residues P4–P5′ of the SARS-CoV Mpro N-terminal autoprocessing site which has the sequence AVLQSGFRK. SARS-CoV Mpro is a key antiviral target.
This peptide contains an N-terminal a 5-carboxytetramethylrhodamine (5-TAMRA), a widely used fluorescent dye which excites at 546 nm and emits at 579 nm and a black hole quencher 2 (BHQ-2) group. This Mpro substrate is used in Mpro inhibition assays. The compound being tested for its inhibitory capacity of Mpro is added alongside [5-TAMRA]/[Lys(BHQ-2)]-CoV, after a set time period, the amount of fluorescence released is read as a percentage inhibition by a plate reader.
The fluorescence from 5-TAMRA is efficiently quenched by resonance energy transfer to the BHQ-2 group when the peptide is intact, however upon cleavage of the peptide by Mpro, 5-TAMRA and BHQ-2 are separated, allowing fluorescence to be detected. This therefore represents a useful tool for investigating Mpro activity.
[5-TAMRA]/[Lys(BHQ-2)]-CoV Main Protease (Mpro) Substrate
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