Fluorogenic substrate for 20S proteasome, other chymotrypsin-like proteases and calpains. Contains a Rhodamine 110 molecule which will fluoresce upon peptide hydrolysis
Catalogue number crb1100533 Molecular Weight 1015.5 Sequence (one letter code)
Sequence (three letter code)
Suc-LLVY-[Rh110]-[D-Pro], Where Rh110 is Rhodamine 110
Purity >95% References
McKinnon et al., (2020). Early-onset impairment of the ubiquitin-proteasome system in dopaminergic neurons caused by α-synuclein. Acta Neuropathol Commun. 8: 17. PMID: 32059750
Huang et al., (2014). Inhibitory Effect of b-AP15 on the 20S Proteasome. Biomolecules. 4(4): 931. PMID: 25317846
Manufactured in: United Kingdom
Fluorogenic substrate peptide of the 20S proteasome. In its intact state this peptide is non-fluorescent, however when Rhodamine fluorophore is released upon hydrolysation, fluorescence can be detected. This peptide is therefore a useful tool for analysing the activity of the 20S proteasome as well as other chymotrypsin-like proteases and calpains. This peptide is also a substrate for chymase, papain, carboxypeptidase Y, proteinase yscE (kexin) and ingensin.
The presence of the D-proline residue on the C terminal of the rhodamine molecule ensures one directional rhodamine cleavage which simplifies fluorescence studies. Rhodamine 110 is a laser grade fluorescent dye
with excitation maxima at 496 nm and emission maxima at 522 nm.