• Description

  • Application Data


Fluorogenic substrate for 20S proteasome, other chymotrypsin-like proteases and calpains. Contains a Rhodamine 110 molecule which will fluoresce upon peptide hydrolysis

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Application Data

Catalogue number crb1100533
Molecular Weight 1015.5
Sequence (one letter code)


Sequence (three letter code)

Suc-LLVY-[Rh110]-[D-Pro], Where Rh110 is Rhodamine 110

Purity >95%

McKinnon et al., (2020). Early-onset impairment of the ubiquitin-proteasome system in dopaminergic neurons caused by α-synuclein. Acta Neuropathol Commun. 8: 17. PMID: 32059750


Huang et al., (2014). Inhibitory Effect of b-AP15 on the 20S Proteasome. Biomolecules. 4(4): 931. PMID: 25317846

Manufactured in: United Kingdom
Data Sheet Material Safety Data Sheet (MSDS)

Fluorogenic substrate peptide of the 20S proteasome. In its intact state this peptide is non-fluorescent, however when Rhodamine fluorophore is released upon hydrolysation, fluorescence can be detected. This peptide is therefore a useful tool for analysing the activity of the 20S proteasome as well as other chymotrypsin-like proteases and calpains. This peptide is also a substrate for chymase, papain, carboxypeptidase Y, proteinase yscE (kexin) and ingensin.

The presence of the D-proline residue on the C terminal of the rhodamine molecule ensures one directional rhodamine cleavage which simplifies fluorescence studies. Rhodamine 110 is a laser grade fluorescent dye
with excitation maxima at 496 nm and emission maxima at 522 nm.


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