SMAC/DIABLO-[Cys(AF647)] AVPIAQKSE-[Cys(AF647)]-acid
Description
Application Data
Description
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SMAC/DIABLO-[Cys(AF647)] is a pro-apoptotic peptide that is derived from the mitochondrial protein known either as Second Mitochondria-Derived Activator of Caspases (Smac) or Direct IAP Binding Protein with low isoelectric point, pI (DIABLO). Contains a C-terminal cysteine linker labelled with Alexa Fluor 647 (AF647), which is a bright, far-red–fluorescent dye with excitation between 594 nm and 633 nm.
Application Data
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Data Sheet Material Safety Data Sheet (MSDS)Catalogue number crb1110326 Molecular Weight 1427.7 Sequence (one letter code) AVPIAQKSE-[Cys(AF647)]-acid Sequence (three letter code) H-Ala-Val-Pro-Ile-Ala-Gln-Lys-Ser-Glu-[Cys(AF647)]-OH
Purity >95% References Adrain et al (2001). Apoptosis-associated release of Smac/DIABLO from mitochondria requires active caspases and is blocked by Bcl-2. EMBO J. PMID: 1172649
Silke et al (2013). Inhibitor of apoptosis (IAP) proteins-modulators of cell death and inflammation. Cold Spring Harb Perspect Biol. PMID: 23378585
Anguiano-Hernandez et al (2007). Smac/DIABLO and colon cancer. Anticancer Agents Med Chem. PMID: 17630921
Manufactured in: United Kingdom SMAC/DIABLO-[Cys(AF647)] is a pro-apoptotic peptide that is derived from the mitochondrial protein known either as Second Mitochondria-Derived Activator of Caspases (Smac) or Direct IAP Binding Protein with low isoelectric point, pI (DIABLO). During apoptosis the mitochondria has increased permeability to Smac/DIABLO, which causes the protein to diffuse into the cytosol. Here, Smac/DIABLO adheres to Inhibitors of apoptosis proteins (IAPs) and prevents them from binding to caspases, which in turn accentuates apoptosis.
This peptide has a C-terminal cysteine linker labelled with Alexa Fluor 647 (AF647), which is a bright, far-red–fluorescent dye with excitation between 594 nm and 633 nm.