[Rhodamine Green]-LifeAct (Abp140 1-17) [Rhodamine Green]-MGVADLIKKFESISKEE-acid
[Rhodamine Green]-LifeAct (Abp140 1-17) contains the fluorophore Rhodamine Green and the 17 amino acid peptide Lifeact, whose high affinity for actin filaments allows it to be used as a cytoskeletal marker.
Catalogue number crb1100899 Molecular Weight 2279.1 Sequence (one letter code) [Rhodamine Green]-MGVADLIKKFESISKEE-acid Sequence (three letter code)
Purity >95% Storage -20°C References
Hall et al (2012) A highly fluorescent DNA toolkit: synthesis and properties of oligonucleotides containing new Cy3, Cy5 and Rhodamine Green monomers. Nucleic Acids Res. 40 (14) e108 PMID: 22495935
Riedl et al (2010) Lifeact: a versatile marker to visualize F-actin. Nat. Methods. 5 (7) 605 PMID: 18536722
Montes-Rodriguez and Kost (2017) Direct Comparison of the Performance of Commonly Employed In Vivo F-actin Markers (Lifeact-YFP, YFP-mTn and YFP-FABD2) in Tobacco Pollen Tubes. Front. Plant Sci. 8 1349 PMID: 28824684
Manufactured in: United Kingdom
[Rhodamine Green]-LifeAct (Abp140 1-17) contains the 17amino acid peptide Lifeact derived from amino acids 1-17 of the Saccharomyces cerevisiae actin binding protein, Abp140. These first 17 amino acids of Abp140 are crucial in allowing Lifeact to localise to actin filaments (F-actin) and therefore it can be used as a cytoskeletal marker. On application, lifeact can be used in the study of plant development and pathogen defence as filamentous actin within the plant’s actin cytoskeleton is important in key processes such as cell division, membrane trafficking and stomatal movements.
The addition of the Rhodamine Green fluorophore, Rhodamine Green allows the location of the LifeAct (Abp140 1-17) to be detected.