Fluorogenic peptide substrate for flavivirus NS3 and NS2B/3 serine proteases
Catalogue number crb1100372 Molecular Weight 1705 Sequence (one letter code) [N-Cbz]-Nle-KRR-[Rh110]-RRK-Nle-[N-Cbz] Sequence (three letter code) [N-Cbz]-Nle-Lys-Arg-Arg-[Rhodamine 110]-Arg-Arg-Lys-Nle-[N-Cbz] Aliase (N-Cbz-Nle-KRR)2-[R110], (N-Cbz-Nle-Lys-Arg-Arg)2-[Rh110] Modifications where N-Cbz is carboxy benzyl, where Rh110 is Rhodamine 110 Purity >95% References
Patkar et al., (2008). Yellow Fever virus NS3 plays an essential role in virus assembly independent of its known enzymatic functions. J Virol. 82(7): 3342. PMID: 18199634
Shiryaev et al., (2007). Cleavage preference distinguishes the two-component NS2B–NS3 serine proteinases of Dengue and West Nile viruses. Biochem J. 401(Pt 3): 743. PMID: 17067286
Manufactured in: United Kingdom
Fluorogenic peptide substrate for flavivirus non-structural 3 (NS3) and non-structural 2B/3 (NS2B/3); highly conserved serine proteases that performs several enzymatic functions critical for virus replication.
The flaviviruses include: zika virus; west nile virus; dengue virus; yellow fever virus, and tick-borne encephalitis. Flaviviruses require proteolytic processing of polyprotein precursors to yield a functional viral particle. This processing is carried out by the two-component protease, consisting NS2B a small integral membrane protein, and NS3, a cytosolic protein.
In its intact state this peptide is not fluorescent, however this substrate peptide is cleaved by NS3 or NS2B serine proteases in two successive steps to release Rhodamine 110. Upon rhodamine 110 fluorophore release fluorescence can then be detected. This peptide therefore allows for the quantification of NS3/ NS2B/3 serine protease activity.
Rhodamine 110 is a widely used red fluorescent probe.