LasB FRET substrate

  • Description

  • Application Data

Description

The C-terminal sequence originally from Pseudomonas aeruginosa elastase LasB, the donor quencher pair EDANS-Dabcyl are attached for FRET analysis.

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Application Data

Catalogue number crb1101629
Molecular Weight 1459.7
Disease Area Antimicrobial molecules
Sequence (three letter code)

[Dabcyl]-Val-Ser-Pro-Ala-Ala-Phe-Ala-Ala-Asp-Leu-[EDANS]

Storage -20°C
Citations

Waite et al., (2012). Pseudomonas aeruginosa possesses two putative type I signal peptidases, LepB and PA1303, each with distinct roles in physiology and virulence. J. bacteriol. 194(17): 4521. PMID: 22730125.

 

Personne et al., (2014). Activity of the type I signal peptidase inhibitor MD3 against multidrug-resistant Gram-negative bacteria alone and in combination with colistin. J. Antimicrob. Chemother. 69(12): 3236. PMID: 25134721.

References

Waite et al., (2012). Pseudomonas aeruginosa possesses two putative type I signal peptidases, LepB and PA1303, each with distinct roles in physiology and virulence. J. bacteriol. 194(17): 4521. PMID: 22730125.

 

Personne et al., (2014). Activity of the type I signal peptidase inhibitor MD3 against multidrug-resistant Gram-negative bacteria alone and in combination with colistin. J. Antimicrob. Chemother. 69(12): 3236. PMID: 25134721.

Material Safety Data Sheet (MSDS)

With the rise of multidrug-resistant bacteria like P. aeruginosa, the hunt for low toxicity inhibitors is paramount. A crucial part of their virulence/life cycle is cleavage of signal peptides. Type I signal peptides have a C-terminal hydrophilic domain containing a signal peptidase cleavage site; commonly found in P. aeruginosa proteins that are cleaved by type I signal petidases (SPases). P. aeruginosa LasB, a type I signal peptide, is a crucial enzyme for bacterial invasion, it degrades elastin and thus aids tissue invasion, without cleavage by a SPase the protein is inactive. This peptide is an ideal candidate for enzymatic assay work in to SPase inhibitor investigations.

Here we provide the substrate LasB sequence with the EDANS-Dabcyl donor quencher pair suitable for SPase inhibitor assays with FRET microscopy analysis. When this peptide is intact, fluorescence from the fluorophore (donor) EDAN is undetectable due to the proximity of the acceptor (quencher) Dabcyl. However, upon cleavage the fluorescence of the EDANS moiety, as measurably by excitation/emission 340/490nm, can be detected due to separation from the Dabcyl quencher. 

LasB FRET substrate

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£270.00
£190.00
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