Histone H3-GGC-amide ARTKQTARKSTGGKAPRKQL-GGC-amide
Histone protein H3 is a core component of the nucleosome, functioning in DNA compaction and availability to transcription machinery. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodelling. Similar to the other core histone, H3 has a globular domain and a flexible N-terminal domain, “histone tail” which can undergo modifications such as acetylation, methylation, phosphorylation and ubiquitination. There is a wealth of data recording these modifications but understanding their significance is not as clear. The first 20 residues (1+20) of H3 N terminal domain are provided here. This sequence has already been used to investigate H3 phosphorylation and methylation events in the cell cycle.
A glycine linker is C-terminally attached as a highly flexibly linker to allow the best pharmacokinetic profiling of the fused domain such as expression, localisation, or activity data. Provision of this H3 sequence with a glycine linker may allow further understanding of histone regulation.
Catalogue number crb1000318 Molecular Weight 2398.3 Sequence (one letter code) ARTKQTARKSTGGKAPRKQL-GGC-amide Sequence (three letter code)
Aliase Abeta1-6 Purity >95% References
Cowell et al., (2002). HP1 and methylation at lysine 9 of histone H3 in animals. Chromosoma, 111: 22. DOI: https://doi.org/10.1007/s00412-002-0182-8.
Hendzel et al., (1997). Mitosis-specific phosphorylation of histone H3 initiates primarily within pericentromeric heterochromatin during G2 and spreads in an ordered fashion coincident with mitotic chromosome condensation. Chromosoma, 106: 348. DOI: https://doi.org/10.1007/s004120050256.
Manufactured in: United Kingdom
Histone H3 peptide, a core histone protein of the nucleosome, with a C-terminal GGC linker.