Formyl-MLF-[Cys(AF488)]
Formyl-MLF-[Cys(AF488)]-amide
Description
Application Data
Description
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Formyl-MLF-[Cys(AF488)], composed of the chemotactic peptide N-formyl-methionine-leucine-phenylalanine and the fluorescent dye, Alexa Fluor 488 (AF488) can be used as a marker of bacterial infections.
Application Data
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Data Sheet Data Sheet Data Sheet Material Safety Data Sheet (MSDS)Catalogue number crb1110384 Molecular Weight 1237.3 Sequence (one letter code) Formyl-MLF-[Cys(AF488)]-amide
Sequence (three letter code) Formyl-Met-Leu-Phe-[Cys(AF488)]-NH2
Purity >95% References Maroteaux and Liu. (2016) Alteration of AMPA Receptor-Mediated Synaptic Transmission by Alexa Fluor 488 and 594 in Cerebellar Stellate Cells. eNeuro. 3(3): ENEURO.0109-15. PMID: 27280156
Pooyan et al (2002) Conjugates Bearing Multiple Formyl-Methionyl Peptides Display Enhanced Binding to but Not Activation of Phagocytic Cells. Bioconjug. Chem. 13(2) 216 PMID: 11906258
Manufactured in: United Kingdom Formyl-MLF-[Cys(AF488)] is composed of the chemotactic peptide: N-formyl-methionine-leucine-phenylalanine. N-formyl-methionine is the N-terminal amino acid present on bacteria and allows the bacteria to interact with phagocytic cells such as macrophages and monocyte-derived dendritic cells through surface formyl-MLF receptors. As a result Formyl-MLF labelled with the fluorescent dye, Alexa Fluor 488 can be used as a marker of bacterial infections. It has also been demonstrated that the use of multiple formyl-MLF moieties can target polymeric drug delivery molecules to phagocytic cells. In addition to Alexa Fluor 488’s application in marking bacterial infections, its properties of being photo-bleaching resistant and having a high quantum yield allow it to carry out its most common use in the visualisation and location of dendritic structures and synapses.