[DABCYL]/[Glu(EDANS)] SARS-CoV-2 3C-like protease (3CLpro) substrate

  • Description

  • Application Data

Description

A potent inhibitor of severe acute respiratory syndrome coronavirus (SARS-CoV) 3C-like protease (3CLpro) activity with the donor quencher pair EDANS-Dabcyl attached for FRET analysis.

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Application Data

Catalogue number crb1101612
Molecular Weight 2079.0
Disease Area Virology
Sequence (three letter code)

[DABCYL]-Lys-Thr-Ser-Ala-Val-Leu-Gln-Ser-Gly-Phe-Arg-Lys-Met-[Glu(EDANS)]-amide

Storage -20°C
Citations

Mahase et al., (2021). Covid-19: Pfizer's paxlovid is 89% effective in patients at risk of serious illness, company report. BMJ. 375: n2713. PMID 34750163.

 

Salwa et al., (2015). SARS-CoV-2 3c-like protease inhibitors of some newly synthesized substituted pyrazoles and substituted pyrimidines based on 1-(3-aminophenyl)-3-(1h-indol-3-yl)prop-2-en-1-one. Int. J. Clin. Pharmacol. Res. 11: 749. DOI: 10.3923/ijp.2015.749.756.

References

Mahase et al., (2021). Covid-19: Pfizer’s paxlovid is 89% effective in patients at risk of serious illness, company report. BMJ. 375: n2713. PMID 34750163.

 

Salwa et al., (2015). SARS-CoV-2 3c-like protease inhibitors of some newly synthesized substituted pyrazoles and substituted pyrimidines based on 1-(3-aminophenyl)-3-(1h-indol-3-yl)prop-2-en-1-one. Int. J. Clin. Pharmacol. Res. 11: 749. DOI: 10.3923/ijp.2015.749.756.

Material Safety Data Sheet (MSDS)

3CLpro are the key enzymes required by coronaviruses to replicate. They cleave polyproteins to form replicase. This makes 3CLpro a drug target for protease inhibitors with particular interest to COVID-19. Synthetic 3CLpro substrates are being generated for their potential to inhibit the protease activity and thus replication cycle of coronaviruses.

When this peptide is intact, fluorescence from the fluorophore (donor) EDAN is undetectable due to the proximity of the acceptor (quencher) Dabcyl. However, upon cleavage the fluorescence of the EDANS moiety, as measurably by excitation/emission 340/490nm, can be detected due to separation from the Dabcyl quencher.  This product was shown to be a potent inhibitor of 3CLpro activity therefore, it has the potential to be a vital tool in the fight against SARS-CoV.

[DABCYL]/[Glu(EDANS)] SARS-CoV-2 3C-like protease (3CLpro) substrate

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£220.00
£320.00
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