Click Pip1

4-Pentynyl-RXRRXRRXRIKILFQNRRMKWKK-amide, X is aminohexanoic acid

  • Description

  • Application Data

Description

Pip1 is a cell-penetrating peptide (CPP) labelled at the N-terminus with an alkyne attachment for ease of reaction with an opposite Click reactive partner (azide).

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Application Data

Catalogue number crb1000127
Molecular Weight 3243.0
Sequence (one letter code)

4-Pentynyl-RXRRXRRXRIKILFQNRRMKWKK-amide, X is aminohexanoic acid

Sequence (three letter code)

4-Pentynyl-Arg-X-Arg-Arg-X-Arg-Arg-X-Arg-Ile-Lys-Ile-Leu-Phe-Gln-Asn-Arg-Arg-Met-Lys-Trp-Lys-Lys-NH2, X is aminohexanoic acid

Modifications X is aminohexanoic acid
Purity >95%
Storage -20°C
References

Ivanova et al., (2008). Improved cell-penetrating peptide-PNA conjugates for splicing redirection in HeLa cells and exon skipping in mdx mouse muscle. Nucleic acids res., 36(20): 6418. doi: 10.1093/nar/gkn671.

Li et al., (2013). Click chemistry in peptide-based drug design. Molecules, 18(8): 9797. doi: 10.3390/molecules18089797.

Manufactured in: United Kingdom
Data Sheet Material Safety Data Sheet (MSDS)

Delivery of peptide nucleic acid (PNA) oligonucleotides to targeted cells is becoming a viable therapeutic strategy by redirecting RNA splicing for conditions like Duchenne muscular dystrophy (DMD). The use of novel cell-penetrating peptides (CPP) has helped create targeted, and increased activity of the PNAs delivered.

PNA internalization peptides (Pip)  is a specifically designed CPP for conjugation to PNAs. Pips were designed to improve PNA activity observed in HeLa cells determined by splicing redirection assays and increased serum stability determined by mass spectrometry. Of the Pip series, Pip1 enters the cell in an energy-dependent manner, primarily via clathrin-dependent endocytosis. Pip1 conjugated to a PNA was determined to be well transported into the cell but is not the most stable against proteolysis. However, this may benefit some cargo depending on the intracellular destination. As a CPP for DMD, the proteolysis of Pip1 was deemed an issue but showed promise for other conjugates in HeLa cells.

Pip1 is labelled at the N-terminus with an alkyne attachment for ease of reaction with an opposite Click reactive partner (azide). Azide-alkyne cycloaddition has become the most popular Click reaction. Alkyne-Pip1 allows various applications, particularly for protein conjugation, modification, and drug delivery.

Click Pip1

Cat No.Pack SizePriceQty.
0.5mg£85.00
1mg£110.00
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