Cathepsin G FRET substrate [5-FAM]/[6-TAMRA]

[5-FAM]-EPFWEDQ-Lys[(6-TAMRA)]-amide

  • Description

  • Application Data

Description

FRET peptide substrate for Cathepsin G, contains the FRET pair 5-FAM and 6-TAMRA

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Application Data

Catalogue number crb1100420
Molecular Weight 1846.7
Sequence (one letter code)

[5-FAM]-EPFWEDQ-Lys[(6-TAMRA)]-amide

Sequence (three letter code)

[5-FAM]-Glu-Pro-Phe-Trp-Glu-Asp-Gln-Lys[(6-TAMRA)]-NH2

Purity >95%
References

Faraday et al., (2013). Cathepsin G-Dependent Modulation of Platelet Thrombus Formation In Vivo by Blood Neutrophils. PLoS One. 8(8): e71447. PMID: 23940756

 

Jun et al., (2018). Caspase-4 activation by a bacterial surface protein is mediated by cathepsin G in human gingival fibroblasts Cell Death Differ. 25(2): 380. PMID: 29077095

Manufactured in: United Kingdom
Data Sheet Material Safety Data Sheet (MSDS)

Substrate peptide for Cathepsin G, a serine protease belonging to the chymotrypsin superfamily which acts as a physiologic regulator of platelet activation and thrombus formation. Cathepsin G also has antimicrobial activity and is involved in chemotaxis, apoptosis, the immune response and inflammation and hydrolysis of extracellular matrix proteins. Cathepsin G can cleave protease activated receptor-4 (PAR4) and is a potential target for novel anti-thrombotic therapies.

This peptide contains the FRET pair: 5-Carboxyfluorescein (5-FAM) and 6-Carboxytetramethylrhodamine (6-TAMRA). 5-Carboxyfluorescein (5-FAM), which is present at the N-terminus of this peptide, is a widely used green fluorescent tag which excites at 495 nm and emits at 517 nm. 6-carboxytetramethylrhodamine (6-TAMRA), which is attached at the C-terminus, is a widely used fluorescent dye which excites at 546 nm and emits at 579 nm. In the intact peptide 6-TAMRA acts as a quencher of the fluorescence of 5-FAM therefore no fluorescence can be detected. However hydrolysis of the peptide between the donor/acceptor pair allows the fluorescence of 5-FAM to be recovered, enabling the quantitative measurement of enzymatic activity.

Fluorescence Resonance Energy Transfer (FRET) peptides are convenient tools for the study of peptidase specificity and enzymatic activity.

Cathepsin G FRET substrate [5-FAM]/[6-TAMRA]

Cat No.Pack SizePriceQty.
1mg£300.00
0.5mg£190.00
0.1mg£170.00
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