C-terminal Sortagging-[Cys(Sulfocyanine3)]

GGG-[C(Sulfocyanine3)]-amide

  • Description

  • Application Data

Description

Acts as a (oligo)glycine nucleophile in the final steps of a sortagging reaction, which results in the yellow fluorescent dye being attached to the C-terminus of the target peptide.

See full description

Application Data

Catalogue number crb1101488
Molecular Weight 1029.3
Sequence (one letter code)

GGG-[C(Sulfocyanine3)]-amide

Sequence (three letter code)

H-Gly-Gly-Gly-[Cys(Sulfocyanine3)]-NH2

Purity >95%
Storage -20°C
References

Strijbis et al (2012) Protein Ligation in Living Cells Using Sortase. Traffic 13(6) 780 PMID: 22348280

Sinisi et al (2012) Development of an Influenza virus Protein Array Using Sortagging Technology. Bioconjug. Chem. 23(6) 1119 PMID: 22594688

Ton-That et al (2000) Anchoring of Surface Proteins to the Cell Wall ofStaphylococcus aureus. J. Biol. Chem. 275(13) 9876 PMID: 10734144

Manufactured in: United Kingdom
Data Sheet Material Safety Data Sheet (MSDS)

This C-terminal Sortagging peptide acts as a (oligo)glycine nucleophile in the final steps of a sortagging protein labelling reaction. This reaction results in the fluorescent moiety being attached to the C-terminus of the target protein or peptide.

A substrate peptide containing the LPXTG motif is recognised and cleaved by the enzyme Sortase A (SrtA) from Staphylococcus aureus. The catalytic cysteine residue in the active site of SrtA, serves as a nucleophile to cleave the peptide bond between threonine and glycine of the substrate peptide. Cleavage results in the formation of a thioacyl intermediate between the substrate peptide and SrtA. This intermediate is then resolved by the N-terminus of this (oligo)glycine nucleophile peptide, resulting in the creation of a new peptide bond that links the substrate peptide to this peptide and its fluorescent dye.  This method of protein labelling is known as sortagging.

This peptide contains Sulfocyanine3, which is a fluorescent yellow dye.

C-terminal Sortagging-[Cys(Sulfocyanine3)]

Cat No.Pack SizePriceQty.
0.1mg£170.00
0.5mg£220.00
1mg£330.00
Bulk Quote

Download Family PDF